[MOS] October 2, 2012

[Zina M Queen]

Modern Optics and Spectroscopy 

Super-resolution fluorescence microscopy

at  the molecular scale 

Joshua Vaughan,

Harvard University



Tuesday, October 2, 2012

12:00 – 1:00 p.m.

From GFP to intracellular sensor dyes, advances in fluorescent probes have been a major driving force in expanding the modern fluorescence microscopy toolkit for biological research. Photoswitchable fluorophores, in particular, have enabled superresolution imaging based on the sequential localization of individual fluorescent molecules (STORM, PALM, etc.), such that researchers may now probe the organization of biological structures at 20-50 nm distance scales (far below the ~200 nm diffraction limit of light) using widely available commercial instrumentation. I will discuss our recent work on fluorescent probes for super-resolution imaging, including robust multi-color imaging at 20-30 nm spatial resolution and a new chemical strategy for fluorophore caging that improves the brightness of the uncaged form by orders of magnitude and achieves 2-10 nm spatial resolution.



Grier Room, MIT Bldg 34-401

Refreshments served after the lecture
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