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<!--StartFragment--><p class="MsoTitle" style="margin-right:-.5in"><b style="mso-bidi-font-weight:
normal"><span style="font-size:8.0pt;mso-bidi-font-size:12.0pt;font-family:
"Times New Roman""><o:p> </o:p></span></b></p><p class="MsoNormal" align="center" style="text-align:center"><font class="Apple-style-span" size="4"><b style="mso-bidi-font-weight:
normal"><span style="letter-spacing: 1.5pt; ">Modern Optics and Spectroscopy</span></b></font><b style="mso-bidi-font-weight:
normal"><span style="font-family: Arial; letter-spacing: 1pt; "><o:p><font class="Apple-style-span" size="4"> </font></o:p></span></b></p><p class="MsoNormal" align="center" style="margin-right:-13.5pt;text-align:center"><b style="mso-bidi-font-weight:normal"><i style="mso-bidi-font-style:normal"><font class="Apple-style-span" size="4">Super-resolution
fluorescence microscopy <o:p></o:p></font></i></b></p><p class="MsoNormal" align="center" style="margin-right:-13.5pt;text-align:center"><b style="mso-bidi-font-weight:normal"><i style="mso-bidi-font-style:normal"><font class="Apple-style-span" size="4">at<span style="mso-spacerun:yes"> </span>the molecular scale</font></i></b><b style="mso-bidi-font-weight:
normal"><i style="mso-bidi-font-style:normal"><span style="letter-spacing: 1pt; "><o:p><font class="Apple-style-span" size="4"> </font></o:p></span></i></b></p><p class="MsoNormal" align="center" style="text-align:center"><font class="Apple-style-span" size="4"><b style="mso-bidi-font-weight:
normal">Joshua Vaughan</b><span style="letter-spacing: 1pt; ">,<o:p></o:p></span></font></p><p class="MsoNormal" align="center" style="text-align:center"><span style="letter-spacing: 1pt; "><font class="Apple-style-span" size="4">Harvard
University<o:p></o:p></font></span></p><p class="MsoNormal" align="center" style="text-align:center"><font class="Apple-style-span" size="4"><span class="Apple-style-span" style="letter-spacing: 1px;"><br></span></font></p><p class="MsoNormal" align="center" style="text-align:center"><span style="letter-spacing: 1pt; "><font class="Apple-style-span" size="4">Tuesday,
October 2, 2012</font></span></p><p class="MsoNormal" align="center" style="text-align:center"><span style="letter-spacing: 1pt; "><font class="Apple-style-span" size="4">12:00 –
1:00 p.m.</font></span></p><p class="MsoNormal" style="text-align:justify"><font class="Apple-style-span" size="4"><span style="font-family:Helvetica;
mso-bidi-font-family:Helvetica">From GFP to intracellular sensor dyes, advances
in fluorescent probes have been a major driving force in expanding the modern
fluorescence microscopy toolkit for biological research. Photoswitchable
fluorophores, in particular, have enabled superresolution imaging based on the
sequential localization of individual fluorescent molecules (STORM, PALM,
etc.), such that researchers may now probe the organization of biological
structures at 20-50 nm distance scales (far below the ~200 nm diffraction limit
of light) using widely available commercial instrumentation. I will discuss our
recent work on fluorescent probes for super-resolution imaging, including
robust multi-color imaging at 20-30 nm spatial resolution and a new chemical
strategy for fluorophore caging that improves the brightness of the uncaged
form by orders of magnitude and achieves 2-10 nm spatial resolution.</span><o:p></o:p></font></p><p class="MsoNormal" style="text-align:justify"><font class="Apple-style-span" size="4"><br></font></p><p class="MsoNormal" align="center" style="text-align:center"><font class="Apple-style-span" size="4">Grier Room, MIT Bldg 34-401<o:p></o:p></font></p><p class="MsoNormal" align="center" style="text-align:center"><font class="Apple-style-span" size="4">Refreshments served after
the lecture</font><font class="Apple-style-span" size="6"><o:p></o:p></font></p>
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