[Oww-Feedback] mistakes in the entry "M9 medium/minimal" (from Markus)

Austin Che austin at csail.mit.edu
Sat Apr 5 16:24:35 EDT 2008


    Thanks for your comment. However, your numbers do not appear
    to agree with other sources. 

    http://www.changbioscience.com/protocols/recipe/M9medium.htm
    http://micro.fhw.oka-pu.ac.jp/protocols/media/m9.html
    
    If you have a source for your numbers or would like to discuss it,
    I would suggest getting an account on openwetware and discussing
    on the appropriate talk page.
    
> I found two mistakes in the entry "M9 medium/minimal"
>
>>1x M9 salts 
>>2mM MgSO4 
>>0.1mM CaCl2 
>>0.4% carbon source (e.g. glycerol, glucose, etc.) 
>>In sterile H2O 
>
> The concentration of 0.1mM CaC12 is to high, it sould be 0.1µM. The same with MgSO4 --> 2µM not 2mM.
> And maybe it would be good to tell the people that they should add low concentrated CaCl12 (e.g. 10ml of 10µM to 1l) to avoid precipitation.

-- 
Austin Che           <austin at csail.mit.edu>          (617)253-5899




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