[Oww-Feedback] mistakes in the entry "M9 medium/minimal" (from Markus)
Austin Che
austin at csail.mit.edu
Sat Apr 5 16:24:35 EDT 2008
Thanks for your comment. However, your numbers do not appear
to agree with other sources.
http://www.changbioscience.com/protocols/recipe/M9medium.htm
http://micro.fhw.oka-pu.ac.jp/protocols/media/m9.html
If you have a source for your numbers or would like to discuss it,
I would suggest getting an account on openwetware and discussing
on the appropriate talk page.
> I found two mistakes in the entry "M9 medium/minimal"
>
>>1x M9 salts
>>2mM MgSO4
>>0.1mM CaCl2
>>0.4% carbon source (e.g. glycerol, glucose, etc.)
>>In sterile H2O
>
> The concentration of 0.1mM CaC12 is to high, it sould be 0.1µM. The same with MgSO4 --> 2µM not 2mM.
> And maybe it would be good to tell the people that they should add low concentrated CaCl12 (e.g. 10ml of 10µM to 1l) to avoid precipitation.
--
Austin Che <austin at csail.mit.edu> (617)253-5899
More information about the Oww-Feedback
mailing list