[Oww-Feedback] mistakes in the entry "M9 medium/minimal" (from Markus)

OpenWetWare Feedback Form on OpenWetWare feedback at openwetware.org
Sat Apr 5 15:10:32 EDT 2008


I found two mistakes in the entry "M9 medium/minimal"

>1x M9 salts 
>2mM MgSO4 
>0.1mM CaCl2 
>0.4% carbon source (e.g. glycerol, glucose, etc.) 
>In sterile H2O 

The concentration of 0.1mM CaC12 is to high, it sould be 0.1µM. The same with MgSO4 --> 2µM not 2mM.
And maybe it would be good to tell the people that they should add low concentrated CaCl12 (e.g. 10ml of 10µM to 1l) to avoid precipitation.

With best regards,
Markus





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