[miso-users] Question about using sashimi_plot

[Yarden Katz]

You can use the version of Sashimi plot that’s built into IGV - all it needs are BAM files. This will not display abundance estimates but will create the plot. If you use the version of sashimi plot that’s part of MISO, you can also just feed it BAM files and annotations and it will make the plot, but won’t display Psi estimates on the right (for that it needs the MISO output files). 

Best,
Yarden

> On May 19, 2020, at 2:37 PM, Nelson Barrientos <nbb5vf at virginia.edu> wrote:
> 
> 
> Good afternoon,
> 
> If I have run a differential splicing analysis with other tools such as LeafCutter whose output gives a table of differential splicing events and enough information to create a GFF file, could I potentially use sashimi_plot to make sashimi plots with these data? Or would it not be possible because the differential splicing analysis was not performed using MISO? Thanks.
> 
> Best regards,
> Nelson Barrientos
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