<html><head><meta http-equiv="content-type" content="text/html; charset=utf-8"></head><body dir="auto">You can use the version of Sashimi plot that’s built into IGV - all it needs are BAM files. This will not display abundance estimates but will create the plot. If you use the version of sashimi plot that’s part of MISO, you can also just feed it BAM files and annotations and it will make the plot, but won’t display Psi estimates on the right (for that it needs the MISO output files). <div><br></div><div>Best,</div><div>Yarden</div><div><div dir="ltr"><br><blockquote type="cite">On May 19, 2020, at 2:37 PM, Nelson Barrientos <nbb5vf@virginia.edu> wrote:<br><br></blockquote></div><blockquote type="cite"><div dir="ltr"><div dir="ltr"><div class="gmail_default" style="font-family:arial,helvetica,sans-serif">Good afternoon,</div><div class="gmail_default" style="font-family:arial,helvetica,sans-serif"><br></div><div class="gmail_default" style="font-family:arial,helvetica,sans-serif">If I have run a differential splicing analysis with other tools such as LeafCutter whose output gives a table of differential splicing events and enough information to create a GFF file, could I potentially use sashimi_plot to make sashimi plots with these data? Or would it not be possible because the differential splicing analysis was not performed using MISO? Thanks.</div><div class="gmail_default" style="font-family:arial,helvetica,sans-serif"><br></div><div class="gmail_default" style="font-family:arial,helvetica,sans-serif">Best regards,</div><div dir="ltr" class="gmail_signature" data-smartmail="gmail_signature"><div dir="ltr"><div><div dir="ltr"><div><div dir="ltr">Nelson Barrientos</div></div></div></div></div></div></div>
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