[miso-users] miso error

[Yarden Katz]

It's a warning and it's normal in most cases, just signaling that it is not indexing genes with only 1 transcript/mRNA.  Those genes by definition have no alternative regions, and so there's no use in quantifying their isoform abundances. 

  --Yarden

On Dec 25, 2012, at 8:49 AM, mykhwab wrote:

> 
> 
> 
> Hi 
> 
> after running this command
> 
>       python index_gff.py --index SE.gff3 indexed_SE_events/
> 
> I have got thtis error..the program is skipping some genes. Why is this so, how can i fix it? 
> 
> 
> 
> 
> Cannot make gene out of AT5G66890
> WARNING: AT5G66900 does not have at least two mRNA/transcript entries with exons. Skipping over...
> Cannot make gene out of AT5G66900
> WARNING: AT5G66910 does not have at least two mRNA/transcript entries with exons. Skipping over...
> Cannot make gene out of AT5G66910
> WARNING: AT5G66920 does not have at least two mRNA/transcript entries with exons. Skipping over...
> Cannot make gene out of AT5G66920
> WARNING: AT5G66940 does not have at least two mRNA/transcript entries with exons. Skipping over...
> Cannot make gene out of AT5G66940
> WARNING: AT5G66950 does not have at least two mRNA/transcript entries with exons. Skipping over...
> Cannot make gene out of AT5G66950
> WARNING: AT5G66960 does not have at least two mRNA/transcript entries with exons. Skipping over...
> Cannot make gene out of AT5G66960
> WARNING: AT5G66970 does not have at least two mRNA/transcript entries with exons. Skipping over...
> 
> 
> 
> Regards
> 
> 
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