[bioundgrd] FW: Looking for a tech experience in downtown Paris ? come investigate mechanosensory transduction in vivo ! MIT France or not, apply !

[Huayra Hnatko]

On 5/23/16, 4:35 PM, "Claire Wyart" claire.wyart at icm-institute.org

>
>Dear Undergrads,
>
>My lab is located in downtown Paris, in the Brain and Spinal cord
>Institute (ICM) within the Pitié-Salpêtrière hospital campus.
>
>My team is composed of 12 people, 6 US citizens, 6 European. We are
>funded by HFSP, NIH and ERC. We are currently looking for a bilingual tech with
>a good experience in molecular biology, possibly in CRISPR/Cas9
>mediated genome editing - we have a very interesting result from a
>screen that we need to follow up on with systematic validation in a
>tissue-specific manner.
>
>Please share the add (attached) to local undergraduate students who may be
>interested in taking a year or two working abroad in a young and
>dynamic lab dedicated to sensory integration in spinal cord. Engineers
>loving to come welcome as well ! ;-)
>
>Thank you much !
>
>Best regards,
>
>Claire Wyart, PhD
>
>------
>
>Tech/Engineer position in the brain and spinal cord institute (ICM), Paris.
>
>
>Type of contract:
>
>12 month contract, renewable long term. Compensation based on previous
>experience.
>
>
>Description of the work:
>
>Work will be performed in the laboratory of Claire Wyart, focusing on
>the optogenetic dissection of the spinal circuits underlying
>locomotion. The new recruit will ideally provide technical assistance
>towards a gene expression screen we recently performed, applying
>CRISPR-mediated gene-editing and in situ hybridization to zebrafish
>embryos and larvae.
>
>
>-     The technician / engineer will focus on the generation and
>molecular characterization of zebrafish mutants using CRISPR
>technology, including:
>
>-       Injection of DNA, RNA, and protein into zebrafish embryos,
>
>-       Screening by fluorescent marker expression and by PCR-based
>genotyping of transgenics and mutants in collaboration with the
>genotyping core facility,
>
>-       Basic molecular biology (DNA extraction, plasmid purification,
>RNA synthesis, RT-PCR, cloning, etc.),
>
>-       Fluorescent in situ hybridization (FISH) in whole mount or in slices,
>
>-       Fluorescent immunohistochemistry,
>
>-       Confocal imaging on live embryos, larvae, and fixed tissue.
>
>
>
>Expertise required:
>
>-       Strong training in molecular biology,
>
>-       zebrafish experience highly appreciated.
>
>
>
>Start date:
>
>Flexible between June and September 2016.
>
>
>
>Please send your CV, motivation letter, and contacts of 3 references
>to: claire.wyart at icm-institute.org
>
>
>
>Key publications:
>
>(1) Wyart, Del Bene, et al., Nature 2009.
>
>(2) Del Bene, Wyart, et al., Science 2010.
>
>(3) Mirat et al., Frontiers in Neural Circuits 2013.
>
>(4) Djenoune et al., Frontiers in Neuroanatomy 2014.
>
>(5) Fidelin et al., Current Biology 2015.
>
>(6) Bohm, Prendergast et al., Nature Communications 2016.
>
>
>
>
>---
>
>"It is better to fail in originality, than to succeed in imitation."
>Herman Melville.
>
>INSTITUT DU CERVEAU ET DE LA MOELLE EPINIERE
>CHU Pitié Salpétrière - 83, bld de l'hôpital - 75013 Paris
>Phone: +33 1 57274310 ; Fax: +33 1 57 27 40 27
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