[Bioundgrd] Fwd: Research Technician Job Opening at Massachusetts General Hospital / Harvard Medical School

[Kathleen Long]

>To Whom it may Concern,
>	As a local New Englander I wanted to pass on to the any interested
>Biology seniors a job opening here at the Pulmonary & Critical Care Research
Laboratory at Massachusetts General Hospital / Harvard Medical School.

>The current availability is as a research technician in Dr. Aaron Waxman's
>lab.  Aaron is just one of several Investigators of the Pulmonary Division
>who in addition to clinical obligations and clinical research, also has a
>strong focus on bench based research.  The focus of Aaron's research entails
>using hyperoxia as a model of acute lung injury.  With the use of transgenic
>mice that overproduce IL-11 or IL-6 in their airways, Aaron has demonstrate
>enhanced survival in 100% oxygen, surviving more than 300% longer than wild
>type animals.  He has shown that this protective effect is associated with a
>decrease in oxygen-induced cell death, DNA fragmentation, and granulocyte
>infiltration, and is mediated only in part by changes in standard pulmonary
>antioxidants.  Currently we are conducting studies to evaluate on how cell
>injury and cell death contributes to this disease process in terms of the
>signaling pathways activated when IL-6-type cytokines protect cells from
>oxygen damage, and investigating how programmed cell death contributes to
>acute lung injury.
>	Technically, the work is conducted both in vitro and in vivo with a
>wide range of investigative techniques being used to look at qualitative and
>quantitative changes in protein, DNA, RNA, as well as overall morphological
>and physiological changes.  While the repertoire of techniques used is
>dependent on what we are investigating, some that I have done in the last
>four years are bacterial culture, tissue culture, western blotting, ELISA,
>immunoprescipitation, immunohistochemistry and fluorescence, RNA and DNA
>isolation, RPA, Comet Assay, PCR, RT-PCR, plasmic preparation and
>transfection, recombinant DNA/Cloning, Animal Husbandry + Colony
>Maintenance, small animal surgery, etc.
>I joined Aaron's research team right after graduation in 2000.  At that
>point the team consisted of just 2 people (Aaron and myself).  In the last
>four years this research team has grown to four people and is set to further
>expand with the funding of a new NIH RO1 grant.  The overall environment of
>the lab is very friendly and fun to work in.  Aaron and the other members of
>his team are very friendly and easily approachable when questions arise.  I
>myself have had the opportunity to contribute on multiple levels on a number
>of different projects.  From being directly involved in designing,
>conducting, and executing experiments, both in vitro and in vivo, to
>assisting in the preparation and formulation of grants, abstracts, and
>journal submissions.  The perks of working for MGH/Harvard are great, from
>exceptional health insurance, tuition reimbursement, and competitive pay.
>I think that this would be an excellent opportunity to allow an interested
>senior to get a unique experience into the research field as well as serve
>as a stepping-stone to higher education if wished.
>Aaron is looking for an individual that can work in a semi-independent
>manner and in collaboration with other members of the research team as well
>as other investigators from other institutions.  The job would entail much
>of the techniques mentioned above, as well as data documentation /
>organization / interpretation / presentation, periodic lab meetings, etc.
>If you happen to know of any interested students could you please ask them
>to pass along to me their resume and cover letter.  I am also attaching the
>job posting which is available on the MGH/Partners website.
>
>Regards
>Nick
>
>  <<Waxman Job Posting.doc>>
>Nicholas Manzo
>Research Technologist
>Massachusetts General Hospital
>Pulmonary & Critical Care Unit
>55 Fruit St, Bulfinch 148
>Boston, MA  02114
>(617) 726-5630
>(617) 724-9650 Fax
>
>