[miso-users] Miso with variable length reads from Ion Proton RNA-seq

[Radovich, Milan]

Thank you Yarden, totally understand, and thanks for the quick response. Much appreciated!

Milan
________________________________________
From: Yarden Katz [yardenny at gmail.com] on behalf of Yarden Katz [yarden at mit.edu]
Sent: Saturday, August 24, 2013 1:11 PM
To: Radovich, Milan
Cc: miso-users at mit.edu
Subject: Re: [miso-users] Miso with variable length reads from Ion Proton RNA-seq

Hi Milan,

It is unfortunately not yet possible.  I recommend trimming/selecting the reads with most common length, or running separately for the major read lengths and pooling the results later (e.g. averaging, or something of that sort.)  It's on our list to add support for this but it isn't that straightforward to implement.

Best, --Yarden



On Aug 24, 2013, at 10:48 AM, Radovich, Milan wrote:

> Dear All,
>    Im wondering if it would be possible to use Miso with variable length reads produced by Ion Proton RNA-seq and mapped using TopHat2? Thanks for consideration of my query!
>
> Best,
> Milan
> _______________________________________________
> miso-users mailing list
> miso-users at mit.edu
> http://mailman.mit.edu/mailman/listinfo/miso-users