[miso-users] Paired end reads with different read length

Marcin Piechota piechota.marcin at gmail.com
Tue Jul 10 09:39:34 EDT 2012


Hi,

I get stuck with a problem while trying to use MISO.
I have paired-end data from SOLiD with different read length 50 for F3 and
35 for F5.
RNA fragments length is about 100 +/- 30, so sometimes reads overlap.

How should I set --read-len and --paired-end parameters?

I would be gratefull for any help,
Marcin Piechota
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