[MOS] TODAY - Modern Optics and Spectroscopy Virtual Seminar with Matthew D. Lew (Wash. Univ. in St. Louis)

[Christine Brooks]

There will be a virtual Modern Optics and Spectroscopy Seminar held today at 12pm. A short Q&A segment will immediately follow the conclusion of the seminar.

Zoom link: https://mit.zoom.us/j/91650528841?pwd=WEZyaXZyYjZrOENLeENpWW9BRU1WUT09
Password: 887819
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Matthew D. Lew
Washington University in St. Louis

“Single-Molecule Orientation Localization Microscopy:
Visualizing Molecular Organization at the Nanoscale”

Visualizing the dynamic movements and interactions between biomolecules remains a challenge, even when using state-of-the-art single-molecule spectroscopic techniques. Towards this goal, we have recently developed new computational imaging methods, called Single-Molecule Orientation Localization Microscopy (SMOLM), for imaging simultaneously the positions and orientations of blinking fluorescent molecules. SMOLM uses engineered dipole-spread functions (the dipole analog of point-spread functions) to improve the orientation-measurement sensitivity of epifluorescence microscopes. One example is the polarized vortex point spread function, which measures molecular orientation and quantifies the 3D rotational diffusion of fluorescent molecules. SMOLM is useful for imaging nanodomains within supported lipid bilayers and the organization of amyloid aggregates at the nanoscale, revealing chemical composition and structural details that cannot be resolved by conventional super-resolved localization microscopy.

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Christine Brooks
Administrative Assistant
Massachusetts Institute of Technology
Department of Chemistry
77 Massachusetts Ave, 6-333
Cambridge, MA 02139
p: 617.253.7239
e: cbrooks at mit.edu<mailto:cbrooks at mit.edu>

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