[MOS] 11/9 - Modern Optics and Spectroscopy Virtual Seminar with Paul French (Imperial College London)

[Christine Brooks]

There will be a virtual Modern Optics and Spectroscopy Seminar held next Tuesday, November 9 at 12pm. A short Q&A segment will immediately follow the conclusion of the seminar.

Zoom link: https://mit.zoom.us/j/97621644771?pwd=WWJGbTZldUJZWkNtNEpuR0Z4SXFxUT09
Password: 614774
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Paul French
Imperial College London (UK)

“Multidimensional fluorescence imaging across the scales”

We are developing multidimensional fluorescence imaging technology, addressing spatial scales from nm – cm,  imaging rates to enable in vivo imaging or automated high content analysis (HCA), and spectroscopic readouts with a particular emphasis on fluorescence lifetime imaging (FLIM) to contrast different molecular species and to map variations in the local fluorophore molecular environment. This includes FLIM applied to read out Förster resonant energy transfer (FRET) in order to assay protein interactions or read out FRET biosensors. We have implemented FLIM in HCA, preclinical endoscopy, clinical intravital microscopy and in vivo optical projection tomography (OPT). To support quantitative FRET measurements with fluorescent proteins as donor/acceptor fluorophores, we have analysed the impact of the slow rotational dephasing compared to their fluorescence lifetime and provided a tool to correct measurements of complex decays.

We are currently developing a modular open source microscopy platform, including HCA, super-resolved microscopy, phase contrast imaging and OPT.  For FLIM HCA we have implemented automated time-gated imaging for multiwell plate assays of protein interactions or cellular metabolism, including the intracellular measurement of KD to quantify protein interactions. To realise super-resolved HCA, we are developing automated multiwell plate easySTORM , providing low-cost, large FOV single molecule localisation microscopy (SMLM) and accelerated SMLM analysis parallelised on a high-performance computing cluster. For clinical applications we have developedhistoSTORM – an implementation of easySTORM with frozen or FFPE tissue sections and clinically-approved antibody labelling.

We aim to implement our multidimensional fluorescence imaging technology using open source software tools for instrument control, data acquisition, analysis and management and to make them practical in lower resource settings. Current open microscopy developments include openFrame, a low-cost, modular, open microscopy platform, a long-range (~200 micron) optical autofocus unit for HCA utilising machine learning, and a single-shot phase contrast technique that can also provide polarisation-resolved imaging. We have also developed a low-cost modular OPT platform that can provide single-shot volumetric imaging, e.g., of live zebrafish.


Christine Brooks
Administrative Assistant
Massachusetts Institute of Technology
Department of Chemistry
77 Massachusetts Ave, 6-333
Cambridge, MA 02139
p: 617.253.7239
e: cbrooks at mit.edu<mailto:cbrooks at mit.edu>

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