[MOS] April 24, 2012

[Zina M Queen]

Modern Optics and Spectroscopy

Chemical imaging- an emerging approach to molecular analysis in cancer pathology
Rohit Bhargava,

University of Illinois at Urbana-Champaign
Tuesday, April 24, 2012
12:00 – 1:00 p.m.


Chemical imaging - an emerging approach to molecular analysis in cancer pathology.  The use of label-free methods for biomedical imaging is rapidly emerging. In particular, the use of spectroscopy to image molecular content is termed chemical imaging. We present here a chemical imaging approach based on mid-infrared spectroscopic imaging that combines the spatial specificity of optical microscopy with the molecular selectivity of vibrational absorption spectroscopy. IR spectroscopic imaging is particularly attractive for the analysis of cells and tissue in that it permits a rapid and simultaneous fingerprinting of inherent biologic content, extraneous materials and metabolic state without the use of labeled probes. Recorded data are related to the structural and functional state of the biological material using computation. We describe the computational strategy and statistical considerations underlying decision-making for this modality. A combination of theory, novel instrumentation and signal processing forms an integrated approach to biochemical analyses. Our laboratory focuses on the analysis of biological materials for histopathology. First, we describe attempts to diagnose and grade cancer in breast and prostate biopsies without human input. Results indicate that a rapid assessment of lesions is possible with high accuracy and their lethality may be predicted using a systems approach to pathology. Applied to engineered 3D tissue models for breast tumors, we show that the imaging technology is useful in rapidly assessing culture quality and that the model systems can act to inform researchers about the involvement of different cell types in cancer progression. Finally, we integrate imaging observations with those from conventional biological experiments to provide a complete view of cancer progression in these systems.


                        Grier Room, MIT Bldg 34-401
Refreshments served after the lecture
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