[CSBi-events] PLEASE JOIN US TUESDAY MORNING 10:30 CSBI SEMINAR

[CSBi events]

Please Join US tomorrow!

                                   	Challenges for Intelligent Image  
Processing in Cryo-Electron Microscopy


Christoph Best
  Dept. of Molecular Structural Biology
  Max Planck Institut fuer Biochemie


10.30am

Tuesday November 21st
First Floor Conference Room

500 Technology Square (NE47)

Abstract

Cryo-electron microscopy enable the imaging of macromolecular  
complexes and cellular structures in a near-natural state at  
molecular resolution. Recent developments in preparation,  
instrumentation, and automation carry the promise of imaging  
molecular structures at sub-nanometer resolutions in their native  
environment, as well as creating molecular maps of the macromolecular  
complexes in the living cell. These advancements pose unique new  
informatics problems in image processing. In particular, methods from  
machine learning and probabilistic modeling will play a large role in  
classifying images, combining them into three-dimensional structures,  
and extracting information from them. I will discuss several examples  
where modern informatics methods may improve electron microscopy such  
as model-free maximum-likelihood classification of projection images,  
particle picking through support vector machines, and 3D  
reconstruction from random projections using the Baum-Welch algorithm  
and Level Set methods.