From csbi-events at mit.edu Tue Apr 4 14:43:35 2006 From: csbi-events at mit.edu (CSBi events) Date: Tue, 4 Apr 2006 14:43:35 -0400 Subject: [CSBi-events] CSBi Speaker Series-4/28/06 (Dr. T. Hughes) Message-ID: Dear CSBi Community, This note simply serves to apprise you of another upcoming CSBi Speaker Series event. Dr. Hughes will be visiting us on Friday, April 28. (please check the CSBi website for a complete seminar list/times/locations) As always, we sincerely hope that you will join us if your schedule permits. Thank you! Dr. Timothy R. Hughes Banting and Best Department of Medical Research Department of Medical and Molecular Genetics Program in Proteomics and Bioinformatics University of Toronto "The Functional Landscape of Gene Expression in Yeast and Mouse" Friday, April 28, 2006 Maclaurin Building (3-270) 3:00 - 4:00 p.m. Light refreshments served at 2:45 p.m. Abstract Research in my laboratory capitalizes on relationships between gene regulation, gene expression, and gene function. In organisms ranging from microbes to mammals, gene expression data has consistently revealed patterns in which groups of functionally-related genes are co-expressed and are presumably driven by dedicated regulators. We are interested in describing how these patterns arise, and in determining the roles of uncharacterized genes that are components of the patterns. I will describe our efforts in solving these problems in both yeast and mouse, using experimental approaches that are applicable on a large scale, and can be readily coupled to computational analysis. Some of these approaches should be transferable to sequenced organisms without well-developed genetic systems. Host: Dr. Christopher B. Burge Biology Department and Biological Engineering Division Contact: Brenda E. Pepe 617.452.3885 Sponsored by CSBi http://csbi.mit.edu/ Annual CSBi Seminar Series in Computational and Systems Biology The entire MIT Community is welcome to attend! -- Brenda E. Pepe Administrative Assistant to Professor Christopher B. Burge Massachusetts Institute of Technology Department of Biology, 68-223 31 Ames Street Cambridge, MA 02139 Tel: 617-452-3885 Fax: 617-452-2936 From csbi-events at mit.edu Wed Apr 12 10:09:07 2006 From: csbi-events at mit.edu (CSBi events) Date: Wed, 12 Apr 2006 10:09:07 -0400 Subject: [CSBi-events] (Reminder) Upcoming CSBi Seminars Message-ID: Dear CSBi Community, This note simply serves to remind you of our upcoming April seminars. Please join us before each seminar for refreshments, if your schedule permits. Seminar abstracts and additional information can be found by accessing the CSBi website at http://csbi.mit.edu/. Thank you! Thursday, April 20, 2006 Dr. Carlos Bustamante, University of California Berkeley "Direct Observation of Substeps Reveals the RNA Unwinding Mechanism of HCV NS3 Helicase" Location and Time Maclaurin Building (4-270) 3:00 - 4:00 p.m. Light refreshments served at 2:45 p.m. and Friday, April 28, 2006 Dr. Timothy R. Hughes,University of Toronto "The Functional Landscape of Gene Expression in Yeast and Mouse" Location and Time Maclaurin Building (3-270) 3:00 - 4:00 p.m. Light refreshments served at 2:45 p.m. -- Brenda E. Pepe Administrative Assistant to Professor Christopher B. Burge Massachusetts Institute of Technology Department of Biology, 68-223 31 Ames Street Cambridge, MA 02139 Tel: 617-452-3885 Fax: 617-452-2936 From csbi-events at mit.edu Tue Apr 18 10:31:01 2006 From: csbi-events at mit.edu (CSBi events) Date: Tue, 18 Apr 2006 10:31:01 -0400 Subject: [CSBi-events] CSBi Seminar Series-4/20/06 (Dr. Bustamante) Message-ID: Dear CSBi Community, This message simply serves to provide you with a reminder of our upcoming CSBi Seminar Series event with Dr. Carlos Bustamante, on Thursday, April 20. Once again, I'd ask that you note, this seminar will be located in building 4, room 270. We sincerely hope that you will join us if your schedule permits. Thank you. Dr. Carlos Bustamante Professor of Molecular and Cell Biology, Physics and Chemistry and Howard Hughes Medical Institute Investigator University of California, Berkeley "Direct Observation of Substeps Reveals the RNA Unwinding Mechanism of HCV NS3 Helicase" Thursday, April 20, 2006 Maclaurin Building (4-270) 3:00 - 4:00 p.m. Light refreshments served at 2:45 p.m. Abstract Helicases are a ubiquitous class of enzymes involved in nearly all aspects of DNA and RNA metabolism. Despite recent progress in understanding their mechanism of action, limited resolution has left inaccessible the detailed mechanisms by which these enzymes couple the rearrangement of nucleic acid structures to the binding and hydrolysis of ATP. Observing individual mechanistic cycles of these motor proteins is central to understanding their cellular functions. Here we follow in real time, at a resolution of two base pairs and 20 ms, the RNA translocation and unwinding cycles of a hepatitis C virus helicase (NS3) monomer. NS3 is a representative superfamily-2 helicase essential for viral replication and therefore a potentially important drug target. We show that the cyclic movement of NS3 is coordinated by ATP in discrete steps of 11?3 base pairs, and that actual unwinding occurs in rapid smaller substeps of 3.6?1.3 base pairs, also triggered by ATP binding, indicating that NS3 might move like an inchworm. This ATP-coupling mechanism is likely to be applicable to other non-hexameric helicases involved in many essential cellular functions. The assay developed here should be useful in investigating a broad range of nucleic acid translocation motors. Host: Dr. Robert T. Sauer Biology Department Contact: Brenda E. Pepe 617.253.6077 Sponsored by CSBi http://csbi.mit.edu/ Annual CSBi Seminar Series in Computational and Systems Biology The entire MIT Community is welcome to attend! -- Brenda E. Pepe Administrative Assistant to Professor Christopher B. Burge Massachusetts Institute of Technology Department of Biology, 68-223 31 Ames Street Cambridge, MA 02139 Tel: 617-452-3885 Fax: 617-452-2936 From csbi-events at mit.edu Wed Apr 26 08:27:37 2006 From: csbi-events at mit.edu (CSBi events) Date: Wed, 26 Apr 2006 08:27:37 -0400 Subject: [CSBi-events] (REMINDER)CSBi Seminar Series-4/28/06 -Dr. Hughes Message-ID: Dear CSBi Community, This note simply serves to remind you of our upcoming CSBi Seminar Series event with Dr. Timothy Hughes, on Friday, April 28. Please note, this seminar will be in our usual location, building 3, room 270. We sincerely hope that you will join us if your schedule permits. Thank you. Dr. Timothy R. Hughes Banting and Best Department of Medical Research Department of Medical and Molecular Genetics Program in Proteomics and Bioinformatics University of Toronto "The Functional Landscape of Gene Expression in Yeast and Mouse" Friday, April 28, 2006 Maclaurin Building (3-270) 3:00 - 4:00 p.m. Light refreshments served at 2:45 p.m. Abstract Research in my laboratory capitalizes on relationships between gene regulation, gene expression, and gene function. In organisms ranging from microbes to mammals, gene expression data has consistently revealed patterns in which groups of functionally-related genes are co-expressed and are presumably driven by dedicated regulators. We are interested in describing how these patterns arise, and in determining the roles of uncharacterized genes that are components of the patterns. I will describe our efforts in solving these problems in both yeast and mouse, using experimental approaches that are applicable on a large scale, and can be readily coupled to computational analysis. Some of these approaches should be transferable to sequenced organisms without well-developed genetic systems. Host: Dr. Christopher B. Burge Biology Department and Biological Engineering Division Contact: Brenda E. Pepe 617.452.3885 Sponsored by CSBi http://csbi.mit.edu/ Annual CSBi Seminar Series in Computational and Systems Biology The entire MIT Community is welcome to attend! -- Brenda E. Pepe Administrative Assistant to Professor Christopher B. Burge Massachusetts Institute of Technology Department of Biology, 68-223 31 Ames Street Cambridge, MA 02139 Tel: 617-452-3885 Fax: 617-452-2936