[CSBi-events] CSBi Seminar - High Performance Deconvolution Imaging

[csbi-events@mit.edu]

High Performance Deconvolution and Effects on Downstream Image Analysis

Hans van der Voort, Ph.D.
10:30am , Thursday, September 29th
McGovern Auditorium, Whitehead Institute

Deconvolution aims to recover the 'true' object distribution from an 
image distorted by, among others, diffraction and noise. The 
resolution measured in terms of the Half Intensity Width (HIW) in 
deconvolved images can be much higher than in the original, under 
ideal conditions near-isotropic sub-100nm HIW values can be achieved. 
However, in confocal or two-photon fluorescence microscopy the 
available signal strengths do not allow such extremely high HIW 
values, but there deconvolution can significantly improve the axial 
resolution, while effectively reducing noise.The introduction in 
recent years of fast Nipkow disk based confocal microscopes has made 
it possible to acquire large 3D-time series. On top of that, the 
number of voxels per 3D image frame is steadily increasing. Since 
deconvolution is generally a compute intensive procedure these two 
ongoing developments result in a rapidly growing demand for high 
performance deconvolution systems. In this talk we will discuss high 
performance deconvolution algorithms and their effect on resolution. 
In addition the effect of deconvolution on the reliability of 
co-localization coefficients will be discussed in detail.

Contact: James G. Evans (jgevans at wi.mit.edu)