[Crib-list] SPEAKER: Jonathan Gootenberg (MIT) | Computational Research in Boston and Beyond Seminar (CRIBB) | Friday, 12/01/2017 | 11:00 AM - 12:00 PM | Building 32, Room 144 (STATA)

[Shirley Entzminger]

 	    Computational Research in Boston and Beyond Seminar
 				(CRIBB)



DATE:		Friday, December 1, 2017

TIME:		11:00 AM to 12:00 Noon    (NOTE Time)

LOCATION:	Building 32, Room 144  (STATA)
 		   32 Vassar Street
 		   Cambridge, MA

 		(Pizza/beverages will be provided at 10:45 AM outside
 		Room 32-144)


TITLE:		Harnessing computationally discovered CRISPR systems for
 		transcriptome engineering and human health



SPEAKER:	Jonathan Gootenberg  (MIT)


ABSTRACT:

CRISPR systems have enabled substantial progress towards the manipulation 
of DNA for studying genetics and gene therapy.  We extend this utility to 
RNA by leveraging the computationally discovered RNA-targeting CRISPR 
systems Cas13 for applications including diagnostics and targeting RNA in 
live cells. We demonstrate that Cas13 can be engineered for RNA knockdown, 
RNA binding, and RNA editing in mammalian cells.  For diagnostic 
applications, we combine Cas13a with isothermal amplification to establish 
a CRISPR-based diagnostic, providing rapid DNA or RNA detection with 
attomolar sensitivity and single-base mismatch specificity.  We use this 
Cas13-based molecular detection platform, termed SHERLOCK, to detect 
specific strains of Zika and Dengue virus, distinguish pathogenic 
bacteria, genotype human DNA, and identify cell-free tumor DNA mutations. 
Our results establish CRISPR-Cas13 as a flexible platform for RNA 
targeting with wide applicability for studying RNA in mammalian cells and 
detecting nucleic acids, and demonstrate the value of computational mining 
for new molecular tools.


===================================================================

Massachusetts Institute of Technology
Cambridge, MA


For information about the Computational Research in Boston and Beyond Seminar 
(CRIBB), please visit....

 			http://math.mit.edu/crib/



===

Shirley A. Entzminger
Administrative Assistant II
Department of Mathematics
Massachusetts Institute of Technology
77 Massachusetts Avenue
Building 2, Room 350A
Cambridge, MA 02139
PHONE:	(617) 253-4347
FAX:	(617) 253-4358
E-mail:	daisymae at math.mit.edu
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