[Crib-list] TODAY.....SPEAKER: Jonathan Gootenberg (MIT) | Computational Research in Boston and Beyond Seminar (CRIBB) | Friday, 12/01/2017 | 11:00 AM - 12:00 PM | Building 32, Room 144 (STATA) (fwd)

[Shirley Entzminger]

 			T O D A Y . . .



 	    Computational Research in Boston and Beyond Seminar
 				(CRIBB)



DATE:		Friday, December 1, 2017

TIME:		11:00 AM to 12:00 Noon    (NOTE Time)

LOCATION:	Building 32, Room 144  (STATA)
 		   32 Vassar Street
 		   Cambridge, MA

 		(Pizza/beverages will be provided at 10:45 AM outside
 		Room 32-144)


TITLE:		Harnessing computationally discovered CRISPR systems for
 		transcriptome engineering and human health



SPEAKER:	Jonathan Gootenberg  (MIT)


ABSTRACT:

CRISPR systems have enabled substantial progress towards the manipulation of 
DNA for studying genetics and gene therapy.  We extend this utility to RNA by 
leveraging the computationally discovered RNA-targeting CRISPR systems Cas13 
for applications including diagnostics and targeting RNA in live cells. We 
demonstrate that Cas13 can be engineered for RNA knockdown, RNA binding, and 
RNA editing in mammalian cells.  For diagnostic applications, we combine Cas13a 
with isothermal amplification to establish a CRISPR-based diagnostic, providing 
rapid DNA or RNA detection with attomolar sensitivity and single-base mismatch 
specificity.  We use this Cas13-based molecular detection platform, termed 
SHERLOCK, to detect specific strains of Zika and Dengue virus, distinguish 
pathogenic bacteria, genotype human DNA, and identify cell-free tumor DNA 
mutations. Our results establish CRISPR-Cas13 as a flexible platform for RNA 
targeting with wide applicability for studying RNA in mammalian cells and 
detecting nucleic acids, and demonstrate the value of computational mining for 
new molecular tools.


===================================================================

Massachusetts Institute of Technology
Cambridge, MA


For information about the Computational Research in Boston and Beyond Seminar 
(CRIBB), please visit....

 			http://math.mit.edu/crib/



===

Shirley A. Entzminger
Administrative Assistant II
Department of Mathematics
Massachusetts Institute of Technology
77 Massachusetts Avenue
Building 2, Room 350A
Cambridge, MA 02139
PHONE:	(617) 253-4347
FAX:	(617) 253-4358
E-mail:	daisymae at math.mit.edu
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