[bioundgrd] Fwd: FAES Bio-Trac Junior Scientist Training Program - starts Sept 2012, lecture & hands-on laboratory
Janice Chang
jdchang at MIT.EDU
Wed Sep 5 14:05:31 EDT 2012
Begin forwarded message:
> From: Mark Nardone <nardonem at mail.nih.gov>
> Date: September 5, 2012 1:59:49 PM EDT
> Subject: FAES Bio-Trac Junior Scientist Training Program - starts Sept 2012, lecture & hands-on laboratory
>
> SPONSORED BY
> FAES (Foundation for The Advanced Education in the Sciences, Inc.)
> at the National Institutes of Health
> Dear Colleague,
>
> The FAES is pleased to announce the 3rd annual Junior Scientist Training Program (JSTP) at the NIH. This nine month program is ideal Post Bacs, fourth year biology majors, recent college graduates or those in the sciences who need to strengthen their laboratory skills.
> JSTP participants will attend seventeen hands-on laboratory sessions (seventy-five contact hours) involving the latest relevant laboratory techniques in molecular biology. These selected methods have been identified by NIH scientists and private industry representatives to be techniques that research technicians use on a day-to-day basis.
>
> Participants would also view online, selected lectures (approx. thirty-five hours) that are part of the current FAES Bio-Trac curriculum offered at the NIH. These lectures, which are taught at a graduate level by area researchers, will cover many areas of biotechnology that will introduce and reinforce the subject matter given in the laboratory. Participating researchers will be available to serve as mentors for interns who participate in the program.
>
> Upon conclusion of the program, participants would receive six graduate credits from the FAES.
> Workshop and registration information can be found at http://www.biotrac.com/pages/Tracs/Trac_Junior_Scientist_Training_Program.html . The registration fee for this program is $4,000.00, with a payment plan being available.
>
> All the best,
>
> Mark
>
>
> Mark Nardone
> FAES/NIH
> Director
> Bio-Trac/CellServ Programs
> Biotechnology Training Contractor
> 301-496-8290
> Bio-Trac, est. in 1985, has provided training to over 14,000 scientists and is open to the scientific community.
> Junior Scientist Pipeline Training Program
> Course Outline
> Monday, September 10, 2012
> Lecture Video 1
> 1. Lab Overview (Dr. Leppert, Ms. Hsu, Ms. Weaver)
> a. Lab Safety
> b. How to Maintain a Laboratory Journal
> c. Laboratory Orientation
> d. Laboratory Protocol and Instruction on the Proper Use of Laboratory Equipment: Pipette Aids and Micropipettors
> Monday, September 17, 2012
> Lecture Video 2
> 2. Basic Tissue Culture (Dr. Nardone)
> a. Quality Control; Mycoplasma
> b. Cross-Contamination & Misidentification
> Monday, September 24, 2012
> Laboratory 1 – 12:00 – 5:00
> 3. Laboratory Safety, Skills and Microscopy (Dr. Leppert, Ms. Gorham)
> a. Practicing Safe Science
> b. Proper Handling and Operation of Lab Equipment
> c. Basic Microscopy
> d. Buffer Preparation (dry lab)
> Monday, October 1, 2012
> Lecture Video 3
> 4. Biotechnology and Cell Culture Formats (Dr. Nardone)
> Monday, October 15, 2012
> Laboratory 2– 12:00 – 5:00
> 5. Mammalian Tissue Culture (Ms. Gorham, Mr. Baker)
> a. Basic Cell and Tissue Culture: culturing and maintaining mammalian cell lines; cell counting
> Monday, October 22, 2012
> Lecture Video 4
> 6. Introduction to Nucleic Acids and Molecular Cloning (Dr. Castora)
> Monday, October 29, 2012
> Lecture Video 5
> 7. Isolation and Characterization of DNA (Dr. Leppert)
> Lecture Video 6
> 8. Isolation and Characterization of RNA (Dr. Leppert)
> Monday, November 5, 2012
> Lecture Video 7
> 9. The Enzymology of Molecular Biology: Restriction Endonucleases and other Pertinent Enzymes (Dr. Castora)
> Monday, November 12, 2012
> Laboratory 3– 12:00 – 5:00
> 10. Nucleic Acid Isolation (Dr. Leppert)
> a. Isolation of Mammalian Genomic DNA
> b. Isolation of Bacterial Plasmid DNA
> c. Isolation of Total Mammalian RNA
> Monday, November 19, 2012
> Lecture Video 8
> 11. The Generation of Recombinant Molecules: Cloning and Library Construction (Dr. Zarlenga)
> a. Construction of Genomic DNA Libraries
> b. cDNA Synthesis
> c. Construction of cDNA Libraries
> Monday, November 26, 2012
> Laboratory 4 – 12:00 – 5:00
> 12. Restriction Digestion/Agarose Gel Electrophoresis (Dr. Leppert)
> a. Restriction Digestion of Plasmid DNA
> b. Restriction Digestion of Mammalian Genomic DNA
> c. Agarose Gel Electrophoresis
>
>
> Monday, December 3, 2012
> Laboratory 5 – 12:00 – 5:00
> 13. Cloning and Bacterial Transformation (Dr. Leppert)
> a. Digestion and Purification of Insert DNA
> b. Cloning Insert DNA into Plasmid Vector
> c. Bacterial Transformation
> Monday, December 10, 2012
> Lecture Video 9 & 10
> 14. Filter Hybridization Techniques (Dr. Leppert)
> a. Blotting of Nucleic Acids; Nucleic Acid Labeling;
> b. Hybridization and Detection Methodologies
> Tuesday, December 11, 2012
> Lecture Video 11
> 15. DNA Microarrays: An Introduction (Dr. Greene)
> Monday, December 17, 2012
> Laboratory 6 – 12:00 – 5:00
> 16. Electrophoresis & Probe Labeling (Dr. Leppert)
> a. Agarose Gel Electrophoresis of Digested DNA from Laboratory 3
> b. DNA Transfer using Southern Method
> c. Probe Labeling
> Tuesday, December 18, 2012
> Laboratory 7 – 12:00 – 5:00
> 17. Filter Hybridization and Chemiluminescent Techniques (Dr. Leppert)
> a. Filter Prehybridization
> b. Filter Hybridization
> c. Chemiluminescent Detection
>
> Monday, January 7, 2013
> Lecture Video 12
> 18. In situ Hybridization: Applications and Techniques (Dr. Blancato)
> Monday, January 14, 2013
> Lecture Video 13
> 19. PCR Methodology: Principles, Optimization and Applications (Dr. Leppert)
> Lecture Video 14
> 20. Real Time PCR (Dr. Kulesh)
> Monday, January 21, 2013
> Laboratory 8 – 12:00 – 5:00
> 20. In Situ Hybridization Techniques (Dr. Blancato, Mr. Baker)
> Monday, January 28, 2013
> Laboratory 9 – 12:00 – 5:00
> 21. PCR/RT-PCR/Real Time PCR (Dr. Kulesh)
> a. Amplification of DNA
> b. Amplification of RNA: Reverse Transcription PCR (RT-PCR)
> c. Real Time and Quantitative PCR
> Monday, February 4, 2013
> Laboratory 10 – 12:00 – 5:00
> 22. PCR/RT-PCR/Real Time PCR Cont’d (Dr. Leppert, Dr. Kulesh)
> Monday, February 11, 2013
> Laboratory 11 – 12:00 – 5:00
> 23. Gene Expression Analysis: Microarrays, Micro RNAs (Ray Manyoky)
> Monday, February 25, 2013
> Laboratory 12 – 12:00 – 5:00
> 24. Gene Expression Analysis: Microarrays, Micro RNAs Cont’d (Qiagen)
> Monday, March 4, 2013
> Lecture Video 15
> 25. Introduction to Proteomics (Dr. Strader)
> Lecture Video 16
> 26. Proteomics: First and Second Dimension Gel Electrophoresis (Dr. Diez)
> Monday, March 11, 2013
> Lecture Video 17
> 27. Cells of the Immune System (Dr. Leitner)
> March 18, 2013
> Laboratory 13 – 12:00 – 5:00
> 28. Proteomics (Dr. Diez)
> a. First Dimension Gel Electrophoresis
> b. Second Dimension Gel Electrophoresis
> Monday, March 25, 2013
> Lecture Video 18
> 29. Immunoassays: Techniques and Applications (Dr. Leitner)
> April 1, 2013
> Monday, Laboratory 14 – 12:00 – 5:00
> 30. Immunoassays: ELISA, Western Analysis, Immunofluorescence (IFA); Fluorescence Activated Cell Sorting (FACS) (Dr. Bergmann, Dr. Leitner)
> a. ELISA
> b. Western Analysis: PAGE, Blotting, Antibody Detection
> c. Immunofluorescence (IFA)
> d. Fluorescence Activated Cell Sorting (FACS) (Demo)
> Monday, April 8, 2013
> Laboratory 15 – 12:00 – 5:00
> 31. Immunoassays: ELISA, Western Analysis, Immunofluorescence (IFA); Fluorescence Activated Cell Sorting (FACS) Cont’d (Dr. Bergmann, Dr. Leitner)
> Monday, April 15, 2013
> Lecture Video 19
> 32. Introduction to Transgenic Technology (Dr. Peters)
> Monday, April 22, 2013
> Lecture Video 20
> 33. Microinjection and Transgenic Production (Dr. Peters)
> Monday, April 29, 2013
> Lecture Video 21
> 34. Protein Bioinformatics (Dr. Natale)
> Monday, May 6, 2013
> Laboratory 16 – 12:00 – 5:00
> 35. Bioinformatics (Dr. Natale)
> a. BLAST, Multiple Sequence Alignments, Pattern and Profile Search, Peptide
> Monday, May 13, 2013
> Lecture Video 22
> 36. Stem Cells: Introduction to Adult and Embryonic Stem Cells (Dr. Bressler)
> Monday, May 20, 2013
> Lecture Video 23
> 37. Introduction to Nanotechnology in Cancer (Dr. Patri)
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