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<p class="MsoNormal"><span style="font-size:15.0pt">There will be a <b><span style="color:red">virtual</span></b><span style="color:red">
</span>Modern Optics and Spectroscopy Seminar held tomorrow<b>, April 12 at 12pm.
</b>A short Q&A segment will immediately follow the conclusion of the seminar.</span><o:p></o:p></p>
<p class="MsoNormal"><span style="font-size:11.0pt"> </span><o:p></o:p></p>
<p class="MsoNormal"><b><i><span style="font-size:15.0pt">Zoom link:<span style="color:red">
<a href="https://mit.zoom.us/j/91650528841?pwd=WEZyaXZyYjZrOENLeENpWW9BRU1WUT09">
<span style="color:red">https://mit.zoom.us/j/91650528841?pwd=WEZyaXZyYjZrOENLeENpWW9BRU1WUT09</span></a><br>
</span></span></i></b><b><span style="font-size:16.0pt">Password: 887819<o:p></o:p></span></b></p>
<p class="MsoNormal"><b><span style="font-size:15.0pt">__________________________</span></b><o:p></o:p></p>
<p class="MsoNormal"><b><span style="font-size:18.0pt"><br>
Matthew D. Lew<br>
</span></b><b><span style="font-size:17.0pt">Washington University in St. Louis</span></b><i><span style="font-size:14.0pt"><br>
<br>
</span></i><b><i><span style="font-size:16.0pt">“Single-Molecule Orientation Localization Microscopy:
<br>
Visualizing Molecular Organization at the Nanoscale”<o:p></o:p></span></i></b></p>
<p class="MsoNormal"><i><br>
Visualizing the dynamic movements and interactions between biomolecules remains a challenge, even when using state-of-the-art single-molecule spectroscopic techniques. Towards this goal, we have recently developed new computational imaging methods, called Single-Molecule
Orientation Localization Microscopy (SMOLM), for imaging simultaneously the positions and orientations of blinking fluorescent molecules. SMOLM uses engineered dipole-spread functions (the dipole analog of point-spread functions) to improve the orientation-measurement
sensitivity of epifluorescence microscopes. One example is the polarized vortex point spread function, which measures molecular orientation and quantifies the 3D rotational diffusion of fluorescent molecules. SMOLM is useful for imaging nanodomains within
supported lipid bilayers and the organization of amyloid aggregates at the nanoscale, revealing chemical composition and structural details that cannot be resolved by conventional super-resolved localization microscopy.<o:p></o:p></i></p>
<p class="MsoNormal"><i><br>
_____________________</i><i><span style="font-size:11.0pt"><br>
</span></i> <o:p></o:p></p>
<p class="MsoNormal"><b><span style="font-size:11.0pt">Christine Brooks</span></b><o:p></o:p></p>
<p class="MsoNormal"><span style="font-size:11.0pt">Administrative Assistant</span><o:p></o:p></p>
<p class="MsoNormal"><span style="font-size:11.0pt">Massachusetts Institute of Technology</span><o:p></o:p></p>
<p class="MsoNormal"><span style="font-size:11.0pt">Department of Chemistry</span><o:p></o:p></p>
<p class="MsoNormal"><span style="font-size:11.0pt">77 Massachusetts Ave, 6-333</span><o:p></o:p></p>
<p class="MsoNormal"><span style="font-size:11.0pt">Cambridge, MA 02139</span><o:p></o:p></p>
<p class="MsoNormal"><span style="font-size:11.0pt">p: 617.253.7239</span><o:p></o:p></p>
<p class="MsoNormal"><span style="font-size:11.0pt">e: <a href="mailto:cbrooks@mit.edu">cbrooks@mit.edu</a></span><o:p></o:p></p>
<p class="MsoNormal"> <o:p></o:p></p>
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