<html xmlns:v="urn:schemas-microsoft-com:vml" xmlns:o="urn:schemas-microsoft-com:office:office" xmlns:w="urn:schemas-microsoft-com:office:word" xmlns:x="urn:schemas-microsoft-com:office:excel" xmlns:m="http://schemas.microsoft.com/office/2004/12/omml" xmlns="http://www.w3.org/TR/REC-html40">
<head>
<meta http-equiv="Content-Type" content="text/html; charset=utf-8">
<meta name="Generator" content="Microsoft Word 15 (filtered medium)">
<style><!--
/* Font Definitions */
@font-face
{font-family:Helvetica;
panose-1:0 0 0 0 0 0 0 0 0 0;}
@font-face
{font-family:"Cambria Math";
panose-1:2 4 5 3 5 4 6 3 2 4;}
@font-face
{font-family:Calibri;
panose-1:2 15 5 2 2 2 4 3 2 4;}
@font-face
{font-family:"Calibri Light";
panose-1:2 15 3 2 2 2 4 3 2 4;}
/* Style Definitions */
p.MsoNormal, li.MsoNormal, div.MsoNormal
{margin:0in;
margin-bottom:.0001pt;
font-size:12.0pt;
font-family:"Calibri",sans-serif;}
h1
{mso-style-priority:9;
mso-style-link:"Heading 1 Char";
margin-top:12.0pt;
margin-right:0in;
margin-bottom:0in;
margin-left:0in;
margin-bottom:.0001pt;
page-break-after:avoid;
font-size:16.0pt;
font-family:"Calibri Light",sans-serif;
color:#2F5496;
font-weight:normal;}
a:link, span.MsoHyperlink
{mso-style-priority:99;
color:#0563C1;
text-decoration:underline;}
a:visited, span.MsoHyperlinkFollowed
{mso-style-priority:99;
color:#954F72;
text-decoration:underline;}
p.msonormal0, li.msonormal0, div.msonormal0
{mso-style-name:msonormal;
mso-margin-top-alt:auto;
margin-right:0in;
mso-margin-bottom-alt:auto;
margin-left:0in;
font-size:11.0pt;
font-family:"Calibri",sans-serif;}
span.Heading1Char
{mso-style-name:"Heading 1 Char";
mso-style-priority:9;
mso-style-link:"Heading 1";
font-family:"Calibri Light",sans-serif;
color:#2F5496;}
p.AbstractSummary, li.AbstractSummary, div.AbstractSummary
{mso-style-name:"Abstract\/Summary";
margin-top:6.0pt;
margin-right:0in;
margin-bottom:0in;
margin-left:0in;
margin-bottom:.0001pt;
font-size:12.0pt;
font-family:"Times New Roman",serif;}
p.p1, li.p1, div.p1
{mso-style-name:p1;
margin:0in;
margin-bottom:.0001pt;
font-size:9.0pt;
font-family:Helvetica;
color:#747487;}
p.p2, li.p2, div.p2
{mso-style-name:p2;
margin:0in;
margin-bottom:.0001pt;
font-size:9.0pt;
font-family:Helvetica;
color:#747487;}
p.endnotebibliography, li.endnotebibliography, div.endnotebibliography
{mso-style-name:endnotebibliography;
mso-margin-top-alt:auto;
margin-right:0in;
mso-margin-bottom-alt:auto;
margin-left:0in;
font-size:11.0pt;
font-family:"Calibri",sans-serif;}
span.EmailStyle23
{mso-style-type:personal;
font-family:"Calibri",sans-serif;
color:windowtext;}
span.EmailStyle24
{mso-style-type:personal;
font-family:"Calibri",sans-serif;
color:windowtext;}
span.EmailStyle25
{mso-style-type:personal;
font-family:"Calibri",sans-serif;
color:windowtext;}
span.EmailStyle26
{mso-style-type:personal;
font-family:"Calibri",sans-serif;
color:windowtext;}
span.EmailStyle27
{mso-style-type:personal;
font-family:"Calibri",sans-serif;
color:windowtext;}
span.EmailStyle28
{mso-style-type:personal;
font-family:"Calibri",sans-serif;
color:windowtext;}
span.EmailStyle29
{mso-style-type:personal;
font-family:"Calibri",sans-serif;
color:windowtext;}
span.EmailStyle30
{mso-style-type:personal;
font-family:"Calibri",sans-serif;
color:windowtext;}
span.EmailStyle31
{mso-style-type:personal;
font-family:"Calibri",sans-serif;
color:windowtext;}
span.EmailStyle32
{mso-style-type:personal;
font-family:"Calibri",sans-serif;
color:windowtext;}
span.EmailStyle33
{mso-style-type:personal;
font-family:"Calibri",sans-serif;
color:windowtext;}
span.EmailStyle34
{mso-style-type:personal;
font-family:"Calibri",sans-serif;
color:windowtext;}
span.EmailStyle35
{mso-style-type:personal;
font-family:"Calibri",sans-serif;
color:windowtext;}
span.EmailStyle36
{mso-style-type:personal;
font-family:"Calibri",sans-serif;
color:windowtext;}
span.EmailStyle37
{mso-style-type:personal;
font-family:"Calibri",sans-serif;
color:windowtext;}
span.EmailStyle38
{mso-style-type:personal;
font-family:"Calibri",sans-serif;
color:windowtext;}
span.apple-converted-space
{mso-style-name:apple-converted-space;}
span.EmailStyle40
{mso-style-type:personal;
font-family:"Calibri",sans-serif;
color:windowtext;}
span.EmailStyle41
{mso-style-type:personal;
font-family:"Calibri",sans-serif;
color:windowtext;}
span.EmailStyle42
{mso-style-type:personal;
font-family:"Calibri",sans-serif;
color:windowtext;}
span.EmailStyle43
{mso-style-type:personal;
font-family:"Calibri",sans-serif;
color:windowtext;}
span.EmailStyle44
{mso-style-type:personal;
font-family:"Calibri",sans-serif;
color:windowtext;}
span.EmailStyle45
{mso-style-type:personal;
font-family:"Calibri",sans-serif;
color:windowtext;}
span.EmailStyle46
{mso-style-type:personal;
font-family:"Calibri",sans-serif;
color:windowtext;}
span.EmailStyle47
{mso-style-type:personal;
font-family:"Calibri",sans-serif;
color:windowtext;}
span.EmailStyle48
{mso-style-type:personal;
font-family:"Calibri",sans-serif;
color:windowtext;}
span.EmailStyle49
{mso-style-type:personal;
font-family:"Calibri",sans-serif;
color:windowtext;}
span.EmailStyle50
{mso-style-type:personal;
font-family:"Calibri",sans-serif;
color:windowtext;}
span.EmailStyle51
{mso-style-type:personal;
font-family:"Calibri",sans-serif;
color:windowtext;}
span.EmailStyle52
{mso-style-type:personal;
font-family:"Calibri",sans-serif;
color:windowtext;}
span.EmailStyle53
{mso-style-type:personal;
font-family:"Calibri",sans-serif;
color:windowtext;}
span.EmailStyle54
{mso-style-type:personal;
font-family:"Calibri",sans-serif;
color:windowtext;}
span.EmailStyle55
{mso-style-type:personal;
font-family:"Calibri",sans-serif;
color:windowtext;}
span.EmailStyle56
{mso-style-type:personal;
font-family:"Calibri",sans-serif;
color:windowtext;}
span.EmailStyle57
{mso-style-type:personal;
font-family:"Calibri",sans-serif;
color:windowtext;}
span.EmailStyle58
{mso-style-type:personal;
font-family:"Calibri",sans-serif;
color:windowtext;}
span.EmailStyle59
{mso-style-type:personal;
font-family:"Calibri",sans-serif;
color:windowtext;}
span.EmailStyle60
{mso-style-type:personal;
font-family:"Calibri",sans-serif;
color:windowtext;}
span.EmailStyle61
{mso-style-type:personal;
font-family:"Calibri",sans-serif;
color:windowtext;}
span.EmailStyle62
{mso-style-type:personal-reply;
font-family:"Calibri",sans-serif;
color:windowtext;}
.MsoChpDefault
{mso-style-type:export-only;
font-size:10.0pt;}
@page WordSection1
{size:8.5in 11.0in;
margin:1.0in 1.0in 1.0in 1.0in;}
div.WordSection1
{page:WordSection1;}
--></style><!--[if gte mso 9]><xml>
<o:shapedefaults v:ext="edit" spidmax="1026" />
</xml><![endif]--><!--[if gte mso 9]><xml>
<o:shapelayout v:ext="edit">
<o:idmap v:ext="edit" data="1" />
</o:shapelayout></xml><![endif]-->
</head>
<body lang="EN-US" link="#0563C1" vlink="#954F72">
<div class="WordSection1">
<p class="MsoNormal"><span style="font-size:15.0pt">There will be a <b><span style="color:red">virtual</span></b><span style="color:red">
</span>Modern Optics and Spectroscopy Seminar held <b>next Tuesday, November 24 at 12pm.
</b>A Q&A segment will immediately follow the conclusion of the seminar.</span><o:p></o:p></p>
<p class="MsoNormal"><span style="font-size:11.0pt"> </span><o:p></o:p></p>
<p class="MsoNormal"><b><i><span style="font-size:15.0pt">Zoom link:</span></i></b>
<b><i><span style="font-size:15.0pt;color:red"><a href="https://mit.zoom.us/j/95525593074?pwd=UGoyUkY4WkFUM0VrS00vVGFtSXZsdz09"><span style="color:red">https://mit.zoom.us/j/95525593074?pwd=UGoyUkY4WkFUM0VrS00vVGFtSXZsdz09</span></a></span></i></b><o:p></o:p></p>
<p class="MsoNormal"><b><span style="font-size:16.0pt">Password: 894959</span></b><o:p></o:p></p>
<p class="MsoNormal"><b><span style="font-size:15.0pt">__________________________</span></b><o:p></o:p></p>
<p class="MsoNormal"><b><span style="font-size:18.0pt"><br>
Dan Oron</span></b><b><span style="font-size:17.0pt"><br>
Weizmann Institute of Science (Israel)</span></b><i><span style="font-size:14.0pt"><br>
<br>
</span></i><b><i><span style="font-size:18.0pt">“Quantum enhanced superresolution microscopy”</span></i></b><o:p></o:p></p>
<p class="MsoNormal"><br>
<i><span style="font-size:13.0pt">Far-field optical microscopy beyond the Abbe diffraction limit, making use of nonlinear excitation (e.g. STED), or temporal fluctuations in fluorescence (PALM, STORM, SOFI) is already a reality. In contrast, overcoming the
diffraction limit using non-classical properties of light is very difficult to achieve due to the difficulty in generating quantum states of light and their inherent fragility. Here, we experimentally demonstrate practical superresolution microscopy based
on quantum properties of light naturally emitted by fluorophores used as markers in fluorescence microscopy. Our approach is based on photon antibunching, the tendency of fluorophores to emit photons one by one rather than in bursts.
<br>
<br>
<o:p></o:p></span></i></p>
<p class="MsoNormal"><i><span style="font-size:13.0pt">Since the non-classical intensity correlations carry higher spatial frequency information, they can be utilized to enhance image resolution. We demonstrate how antibunching can improve the resolution capabilities
of image-scanning confocal microscopy in all three dimensions<sup>1</sup>, and show how the synthesis of classical and quantum information enables us to apply algorithmic resolution augmentation methods<sup>2</sup>. Finally, we show that these methods are
compatible with currently developed SPAD arrays, serving as small single-photon imaging detectors, and thus require little infrastructure investment<sup>3</sup>.
<br>
<br>
<o:p></o:p></span></i></p>
<p class="MsoNormal"><i><span style="font-size:13.0pt">Notably, the use of photon antibunching readily lends itself to quantitative imaging modalities since the degree of antibunching is intimately related to the emitter density. Potential approaches to such
quantitative superresolution microscopy using a simple confocal microscope setup will be discussed.<o:p></o:p></span></i></p>
<p class="MsoNormal"><i><span style="font-size:13.0pt"><o:p> </o:p></span></i></p>
<p class="MsoNormal"><i>References<o:p></o:p></i></p>
<p class="MsoNormal"><i>[1] R. Tenne et al., “Super-resolution enhancement by quantum image scanning microscopy”, Nature Photonics 13, 116 (2019).<o:p></o:p></i></p>
<p class="MsoNormal"><i>[2] U. Rossman et al., “Rapid quantum image scanning microscopy by joint sparse reconstruction”, Optica 6, 1290 (2019).<o:p></o:p></i></p>
<p class="MsoNormal"><i>[3] G. Lubin et al., “Quantum correlation measurement with single photon avalanche diode arrays”, Optics Express 27, 32863 (2019).</i> <br>
<br>
<o:p></o:p></p>
<p class="MsoNormal"> <o:p></o:p></p>
<p class="MsoNormal"><b><span style="font-size:11.0pt">Christine Brooks</span></b><o:p></o:p></p>
<p class="MsoNormal"><span style="font-size:11.0pt">Administrative Assistant</span><o:p></o:p></p>
<p class="MsoNormal"><span style="font-size:11.0pt">Massachusetts Institute of Technology</span><o:p></o:p></p>
<p class="MsoNormal"><span style="font-size:11.0pt">Department of Chemistry</span><o:p></o:p></p>
<p class="MsoNormal"><span style="font-size:11.0pt">77 Massachusetts Ave, 6-333</span><o:p></o:p></p>
<p class="MsoNormal"><span style="font-size:11.0pt">Cambridge, MA 02139</span><o:p></o:p></p>
<p class="MsoNormal"><span style="font-size:11.0pt">p: 617.253.7239</span><o:p></o:p></p>
<p class="MsoNormal"><span style="font-size:11.0pt">e: <a href="mailto:cbrooks@mit.edu">cbrooks@mit.edu</a></span><o:p></o:p></p>
<p class="MsoNormal"> <o:p></o:p></p>
</div>
</body>
</html>