<html xmlns:o="urn:schemas-microsoft-com:office:office" xmlns:w="urn:schemas-microsoft-com:office:word" xmlns:m="http://schemas.microsoft.com/office/2004/12/omml" xmlns="http://www.w3.org/TR/REC-html40">
<head>
<meta http-equiv="Content-Type" content="text/html; charset=utf-8">
<meta name="Title" content="">
<meta name="Keywords" content="">
<meta name="Generator" content="Microsoft Word 15 (filtered medium)">
<style><!--
/* Font Definitions */
@font-face
{font-family:"Cambria Math";
panose-1:2 4 5 3 5 4 6 3 2 4;}
@font-face
{font-family:Calibri;
panose-1:2 15 5 2 2 2 4 3 2 4;}
@font-face
{font-family:"Shree Devanagari 714";
panose-1:2 0 6 0 0 0 0 0 0 0;}
/* Style Definitions */
p.MsoNormal, li.MsoNormal, div.MsoNormal
{margin:0in;
margin-bottom:.0001pt;
font-size:12.0pt;
font-family:"Calibri",sans-serif;}
a:link, span.MsoHyperlink
{mso-style-priority:99;
color:#0563C1;
text-decoration:underline;}
a:visited, span.MsoHyperlinkFollowed
{mso-style-priority:99;
color:#954F72;
text-decoration:underline;}
span.EmailStyle17
{mso-style-type:personal-compose;
font-family:"Calibri",sans-serif;
color:windowtext;}
span.msoIns
{mso-style-type:export-only;
mso-style-name:"";
text-decoration:underline;
color:teal;}
.MsoChpDefault
{mso-style-type:export-only;
font-family:"Calibri",sans-serif;}
@page WordSection1
{size:8.5in 11.0in;
margin:1.0in 1.0in 1.0in 1.0in;}
div.WordSection1
{page:WordSection1;}
--></style>
</head>
<body bgcolor="white" lang="EN-US" link="#0563C1" vlink="#954F72">
<div class="WordSection1">
<p class="MsoNormal"><span style="font-family:"Shree Devanagari 714",sans-serif">There will be a Special Seminar hosted by the Laser Biomedical Research Center on
<b>Thursday, November 8 at 4:30pm</b> in the Moore Room (6-321):<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-family:"Shree Devanagari 714",sans-serif"><o:p> </o:p></span></p>
<p class="MsoNormal"><b><span style="font-size:13.0pt;font-family:"Shree Devanagari 714",sans-serif">Ben Ovryn, Ph.D.<br>
<i>“Single molecule tracking and super-resolution imaging of glycoconjugates on membranes, nanotubes and filopodia in live cells”</i><o:p></o:p></span></b></p>
<p class="MsoNormal"><i><span style="font-family:"Shree Devanagari 714",sans-serif"><o:p> </o:p></span></i></p>
<p class="MsoNormal"><i><span style="font-family:"Shree Devanagari 714",sans-serif">The surface of eukaryotic cells is covered with a dense layer of glycans that affect numerous physiological processes and aberrant cell-surface glycosylation has been implicated
in cancer, inflammation and other diseases. Using a combination of bioorthogonal click reactions, super-resolution imaging and single molecule tracking, we are able to observe the dynamics of cell-surface glycans on live cells with high spatial and temporal
resolution. Our custom microscope also implements a form of confocal interference microscopy, based upon laser feedback interferometry, to simultaneously measure the topography of the membrane. We now seek to experimentally verify the predictions of our model
which incorporates the energetics that govern the nucleation, growth and disassembly of early integrin adhesions in the presence of long glycan molecules. Our experimental approach has enabled the visualization of dynamic tunneling nanotubes connections between
cells and the tracking of glycosylated receptors along the surface of these membranes.<o:p></o:p></span></i></p>
<p class="MsoNormal"><span style="font-size:11.0pt"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt"><o:p> </o:p></span></p>
<p class="MsoNormal"><b><span style="font-size:11.0pt">Christine Brooks</span></b><span style="font-size:11.0pt"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt">Administrative Assistant<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt">Massachusetts Institute of Technology<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt">Department of Chemistry<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt">77 Massachusetts Ave, 6-333<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt">Cambridge, MA 02139<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt">p: 617.253.7239<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt">e: <a href="mailto:cbrooks@mit.edu"><span style="color:#0563C1">cbrooks@mit.edu</span></a><o:p></o:p></span></p>
<p class="MsoNormal"><o:p> </o:p></p>
</div>
</body>
</html>