<html xmlns:o="urn:schemas-microsoft-com:office:office" xmlns:w="urn:schemas-microsoft-com:office:word" xmlns:m="http://schemas.microsoft.com/office/2004/12/omml" xmlns="http://www.w3.org/TR/REC-html40">
<head>
<meta http-equiv="Content-Type" content="text/html; charset=utf-8">
<meta name="Title" content="">
<meta name="Keywords" content="">
<meta name="Generator" content="Microsoft Word 15 (filtered medium)">
<style><!--
/* Font Definitions */
@font-face
{font-family:"Cambria Math";
panose-1:2 4 5 3 5 4 6 3 2 4;}
@font-face
{font-family:Calibri;
panose-1:2 15 5 2 2 2 4 3 2 4;}
/* Style Definitions */
p.MsoNormal, li.MsoNormal, div.MsoNormal
{margin:0in;
margin-bottom:.0001pt;
font-size:12.0pt;
font-family:"Calibri",sans-serif;}
a:link, span.MsoHyperlink
{mso-style-priority:99;
color:#0563C1;
text-decoration:underline;}
a:visited, span.MsoHyperlinkFollowed
{mso-style-priority:99;
color:#954F72;
text-decoration:underline;}
span.EmailStyle17
{mso-style-type:personal;
font-family:"Calibri",sans-serif;
color:windowtext;}
span.EmailStyle18
{mso-style-type:personal;
font-family:"Calibri",sans-serif;
color:windowtext;}
span.EmailStyle19
{mso-style-type:personal;
font-family:"Calibri",sans-serif;
color:windowtext;}
span.EmailStyle20
{mso-style-type:personal;
font-family:"Calibri",sans-serif;
color:windowtext;}
span.EmailStyle21
{mso-style-type:personal-reply;
font-family:"Calibri",sans-serif;
color:windowtext;}
span.msoIns
{mso-style-type:export-only;
mso-style-name:"";
text-decoration:underline;
color:teal;}
.MsoChpDefault
{mso-style-type:export-only;
font-size:10.0pt;}
@page WordSection1
{size:8.5in 11.0in;
margin:1.0in 1.0in 1.0in 1.0in;}
div.WordSection1
{page:WordSection1;}
--></style>
</head>
<body bgcolor="white" lang="EN-US" link="#0563C1" vlink="#954F72">
<div class="WordSection1">
<p class="MsoNormal"><span style="font-size:14.0pt">There will be a Modern Optics and Spectroscopy Seminar held tomorrow,
<b>Tuesday, March 6 at 12pm </b>in <b><span style="color:red">NE47-189 </span></b></span><o:p></o:p></p>
<p class="MsoNormal"><b><span style="font-size:14.0pt;color:red"> </span></b><o:p></o:p></p>
<p class="MsoNormal"><b><span style="font-size:14.0pt;color:red">**Please note location**</span></b><o:p></o:p></p>
<p class="MsoNormal"><b><span style="font-size:14.0pt"> </span></b><o:p></o:p></p>
<p class="MsoNormal"><b><span style="font-size:14.0pt">Joerg Bewersdorf</span></b><o:p></o:p></p>
<p class="MsoNormal"><i><span style="font-size:14.0pt">Yale University</span></i><o:p></o:p></p>
<p class="MsoNormal"><span style="font-size:14.0pt"> </span><o:p></o:p></p>
<p class="MsoNormal"><b><i><span style="font-size:14.0pt">“3D and live-cell multicolor super-resolution microscopy for cell biological research”<o:p></o:p></span></i></b></p>
<p class="MsoNormal"><span style="font-size:14.0pt"> </span><o:p></o:p></p>
<p class="MsoNormal"><i><span style="font-size:14.0pt">Cell biological research relies heavily on the capabilities of light microscopes to resolve structures or processes of interest [1]. My research group is developing super-resolution technology that pushes
these capabilities in particular with respect to 3D resolution, speed, live-cell compatibility and depth penetration. In this presentation, I will report on my lab’s recent progress on imaging whole cells at sub-20 nm 3D resolution and on live-cell imaging
at 50 nm resolution and below. I will summarize published work [2, 3] as well as present recent, unpublished results. Technology and method development will be present in the context of cell biological applications and discoveries.<br>
<br>
<o:p></o:p></span></i></p>
<p class="MsoNormal"><i>[1] D. Baddeley, J. Bewersdorf “Biological Insight from Super-Resolution Microscopy: What We Can Learn from Localization-Based Images”, Annu. Rev. Biochem 2018, in press <a href="http://www.annualreviews.org/doi/abs/10.1146/annurev-biochem-060815-014801">http://www.annualreviews.org/doi/abs/10.1146/annurev-biochem-060815-014801</a><br>
<br>
<o:p></o:p></i></p>
<p class="MsoNormal"><i>[2] F. Huang, G. Sirinakis, E.S. Allgeyer, L.K. Schroeder, W.C. Duim, E.B. Kromann, T. Phan, F.E. Rivera-Molina, J.R. Myers, I. Irnov, M. Lessard, Y. Zhang, M.A. Handel, C. Jacobs-Wagner, C.P. Lusk, J.E. Rothman, D.K. Toomre, M.J. Booth,
J. Bewersdorf “Ultra-high resolution 3D imaging of whole cells”, Cell 2016 <a href="http://www.cell.com/cell/fulltext/S0092-8674(16)30745-0">http://www.cell.com/cell/fulltext/S0092-8674(16)30745-0</a><br>
<br>
<o:p></o:p></i></p>
<p class="MsoNormal"><i>[3] F. Bottanelli, E.B. Kromann, E.S. Allgeyer, R.S. Erdmann, S. Wood Baguley, G. Sirinakis, A. Schepartz, D. Baddeley, D.K. Toomre, J.E. Rothman, J. Bewersdorf “Two-colour live-cell nanoscale imaging of intracellular targets”, Nature
Communication 2016 <a href="https://www.nature.com/articles/ncomms10778">https://www.nature.com/articles/ncomms10778</a><o:p></o:p></i></p>
<p class="MsoNormal"><span style="font-size:11.0pt"><br>
_____________________________________________________________________<br>
<br>
</span><o:p></o:p></p>
<p class="MsoNormal">Refreshments will be served immediately following the seminar<o:p></o:p></p>
<p class="MsoNormal"><span style="font-size:11.0pt"> </span><o:p></o:p></p>
<p class="MsoNormal"><span style="font-size:11.0pt"> </span><o:p></o:p></p>
<p class="MsoNormal"><b><span style="font-size:11.0pt">Christine Brooks</span></b><o:p></o:p></p>
<p class="MsoNormal"><span style="font-size:11.0pt">Administrative Assistant</span><o:p></o:p></p>
<p class="MsoNormal"><span style="font-size:11.0pt">Massachusetts Institute of Technology</span><o:p></o:p></p>
<p class="MsoNormal"><span style="font-size:11.0pt">Department of Chemistry</span><o:p></o:p></p>
<p class="MsoNormal"><span style="font-size:11.0pt">77 Massachusetts Ave, 6-333</span><o:p></o:p></p>
<p class="MsoNormal"><span style="font-size:11.0pt">Cambridge, MA 02139</span><o:p></o:p></p>
<p class="MsoNormal"><span style="font-size:11.0pt">p: 617.253.7239</span><o:p></o:p></p>
<p class="MsoNormal"><span style="font-size:11.0pt">e: <a href="mailto:cbrooks@mit.edu">cbrooks@mit.edu</a></span><o:p></o:p></p>
<p class="MsoNormal"> <o:p></o:p></p>
</div>
</body>
</html>