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<p class="MsoTitle" style="text-align: center; margin-right: -0.5in;"><b><span style="font-family: 'Times New Roman'; font-size: 14px;">Seminar on<o:p></o:p></span></b></p>
<p class="MsoTitle" style="text-align: center; margin-right: -0.5in;"><b><span style="font-family: 'Times New Roman'; font-size: 14px;"> </span></b><b><span style="font-family: 'Times New Roman'; font-size: 14px;"> </span></b><b style="font-size: 14px;"><span style="letter-spacing: 1.5pt;">Modern
Optics and Spectroscopy</span></b></p>
<p class="MsoNormal" style="text-align: center;"><b><span style="font-family: Arial; letter-spacing: 1pt; font-size: 14px;"> </span></b></p>
<p class="MsoNormal" align="center" style="text-align:center"><b><i><span style="letter-spacing: 1pt; font-size: 14px;">Quantitative Phase Imaging in Biomedicine</span></i></b><b><i><span style="letter-spacing: 1pt; font-size: 14px;"> </span></i></b></p>
<p class="MsoNormal" align="center" style="text-align:center"><b><span style="letter-spacing: 1pt; font-size: 14px;">Gabriel Popescu,<o:p></o:p></span></b></p>
<p class="MsoNormal" align="center" style="text-align:center"><span style="letter-spacing: 1pt; font-size: 14px;">University of Illinois at Urbana-Champaign<o:p></o:p></span></p>
<p class="MsoNormal" align="center" style="text-align:center"><span style="letter-spacing: 1pt; font-size: 14px;"> </span></p>
<p class="MsoNormal" align="center" style="text-align:center"><span style="letter-spacing: 1pt; font-size: 14px;">Tuesday, November 3, 2015<o:p></o:p></span></p>
<p class="MsoNormal" align="center" style="text-align:center"><span style="letter-spacing: 1pt;"><span style="font-size: 14px;">12:00 – 1:00 p.m.</span><font size="6"><o:p></o:p></font></span></p>
<p class="MsoNormal" style="text-align:justify"><span style="font-size: 14px;">Quantitative phase imaging (QPI) is emerging as an exciting,
<i style="mso-bidi-font-style:normal">label-free</i> alternative to studying cells and tissues. Unlike fluorescence imaging, QPI is not limited by photobleaching and photoxicity and, thus, can monitor cell function over arbitrary time scales, from milliseconds
to many days. We have developed Spatial Light Interference microscopy (SLIM) as a highly sensitive QPI method. Due to its sub-nanometer pathlength sensitivity, SLIM enables interesting structure and dynamics studies. I will review some of the latest demonstration
of SLIM as a label-free tissue scanner and tool for cancer diagnosis and prognosis in unstained biopsies.<span style="mso-spacerun:yes">
</span>Because it operates without labels and at low illumination power densities, QPI is potentially an ideal option for studying embryo development. However, this application comes with a new set of challenges due to the large (2-300s microns thick), highly
inhomogeneous objects of interest, which generate strong multiple scattering. We found that, for this goal, measuring derivatives of the phase rather than the phase itself, suppresses much of the scattering background. Our new method, Gradient Interference
Microscopy (GIM), allows for imaging embryos over several days and providing information about their viability. I will present our new results on using GIM for studying bovine embryos.<o:p></o:p></span></p>
<p class="MsoNormal"><o:p style="font-size: 14px;"> </o:p></p>
<p class="MsoNormal" align="center" style="text-align:center"><span style="font-size: 14px;">Grier Room, MIT Bldg. 34-401<o:p></o:p></span></p>
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<p class="MsoNormal" align="center" style="text-align:center"><span style="font-size: 14px;">Refreshments served after the lecture</span><span style="font-size: 16pt;"><o:p></o:p></span></p>
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