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</b></font></p>
<p class="MsoNormal" align="center" style="text-align:center"><font class="Apple-style-span" size="4"><b style="mso-bidi-font-weight:
normal"><span style="letter-spacing: 1.5pt; ">Modern Optics and Spectroscopy</span></b><span style="font-family:Arial;
mso-bidi-font-family:"Times New Roman";letter-spacing:1.5pt"><o:p></o:p></span></font></p>
<p class="MsoNormal" align="center" style="text-align:center"><b style="mso-bidi-font-weight:
normal"><span style="font-family: Arial; letter-spacing: 1pt; "><o:p><font class="Apple-style-span" size="4"> </font></o:p></span></b></p>
<p class="MsoNormal" align="center" style="text-align:center"><b><span style="color: black; "><font class="Apple-style-span" size="4">Protein conformational dynamics probed by multidimensional infrared spectroscopy<o:p></o:p></font></span></b></p>
<p class="MsoNormal" align="center" style="text-align:center"><b style="mso-bidi-font-weight:
normal"><i style="mso-bidi-font-style:normal"><span style="letter-spacing: 1pt; "><o:p><font class="Apple-style-span" size="4"> </font></o:p></span></i></b></p>
<p class="MsoNormal" align="center" style="text-align:center"><font class="Apple-style-span" size="4"><b style="mso-bidi-font-weight:
normal">Kevin Jones</b><span style="letter-spacing: 1pt; ">,<o:p></o:p></span></font></p>
<p class="MsoNormal" align="center" style="text-align:center"><span style="letter-spacing: 1pt; "><font class="Apple-style-span" size="4">Massachusetts Institute of Technology</font></span></p>
<p class="MsoNormal" align="center" style="text-align:center"><span class="Apple-style-span" style="letter-spacing: 1px; font-size: large; ">Tuesday, April 17, 2012</span></p>
<p class="MsoNormal" align="center" style="text-align:center"><span class="Apple-style-span" style="font-size: large; "><br>
<span class="Apple-style-span" style="letter-spacing: 1px;">12:00-1:00 p.m.</span></span><span class="Apple-style-span" style="font-family: Times; font-size: large; "> </span></p>
<p class="MsoNormal" style="text-align:justify"><font class="Apple-style-span" size="4">Two dimensional infrared spectroscopy (2D IR), is advantageous for the study of protein and peptide structure and dynamics because it combines ps time resolution with the
conformational sensitivity of vibrational spectroscopy and the increased information content provided by multidimensional nonlinear techniques. We have paired amide I 2D IR with a temperature-jump (T-jump) to reveal the nanosecond to millisecond conformational
dynamics of two proteins:<span style="mso-spacerun:yes"> </span>the<span style="mso-spacerun:yes">
</span>β-hairpin peptide Trpzip2 (TZ2) and the insulin dimer.<span style="mso-spacerun:yes">
</span>To provide site-specific information, two isotopologues of TZ2 were synthesized to reveal changes in the mid-strand and the β-turn region.<span style="mso-spacerun:yes">
</span>The T-jump reveals two distinct timescales:<span style="mso-spacerun:yes">
</span>a <10 ns solvation response and a 1-2 µs time scale for activated disordering of β-turn structures.<span style="mso-spacerun:yes">
</span>The experimental results are interpreted with spectral modeling of MD simulations, which allows us to kinetically model the shifting conformations of the heterogeneous ensemble.<span style="mso-spacerun:yes">
</span>For insulin, the T-jump induces a dissociation process.<span style="mso-spacerun:yes">
</span>Multiple timescales are observed, suggesting the presence of multiple states.<span style="mso-spacerun:yes">
</span>The observed timescales are attributed to unfolding and dissociation processes.</font></p>
<p class="MsoNormal" align="center" style="text-align:center"><font class="Apple-style-span" size="4">Grier Room, MIT Bldg 34-401 </font></p>
<p class="MsoNormal" align="center" style="text-align:center"><font class="Apple-style-span" size="4">Refreshments served after the lecture</font><font class="Apple-style-span" size="6"><o:p></o:p></font></p>
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