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<!--StartFragment--><div style="margin-top: 0in; margin-right: -0.5in; margin-left: 1.5in; margin-bottom: 0.0001pt; text-align: left; text-indent: 0.5in; "><b style="mso-bidi-font-weight:
normal"><span style="letter-spacing: 1.5pt; "><font class="Apple-style-span" size="4"><span class="Apple-tab-span" style="white-space:pre"> </span>Modern Optics and Spectroscopy</font></span></b></div><p class="MsoNormal" align="center" style="text-align:center"><b style="mso-bidi-font-weight:
normal"><span style="font-family: Arial; letter-spacing: 1pt; "><o:p><font class="Apple-style-span" size="4"> </font></o:p></span></b></p><p class="MsoBodyText"><span style="font-family:Times;mso-fareast-font-family:
"ＭＳ 明朝""><font class="Apple-style-span" size="4"><span class="Apple-tab-span" style="white-space:pre"> </span>Dynamic monitoring of engineered tissue development using non-linear
spectroscopic imaging</font></span></p><p class="MsoNormal" align="center" style="text-align:center"><font class="Apple-style-span" size="4"><b style="mso-bidi-font-weight:
normal">Irene Georgakoudi</b><span style="letter-spacing: 1pt; ">,<o:p></o:p></span></font></p><p class="MsoNormal" align="center" style="text-align:center"><span style="letter-spacing: 1pt; "><font class="Apple-style-span" size="4">Tufts
University<o:p></o:p></font></span></p><p class="MsoNormal" align="center" style="text-align:center"><span style="letter-spacing: 1pt; "><o:p><font class="Apple-style-span" size="4"> </font></o:p></span></p><p class="MsoNormal" align="center" style="text-align:center"><span style="letter-spacing: 1pt; "><font class="Apple-style-span" size="4">Tuesday,
December 6, 2011<o:p></o:p></font></span></p><p class="MsoNormal" align="center" style="text-align:center"><span style="letter-spacing: 1pt; "><o:p><font class="Apple-style-span" size="4"> </font></o:p></span></p><p class="MsoNormal" align="center" style="text-align:center"><span style="letter-spacing: 1pt; "><font class="Apple-style-span" size="4">12:00 –
1:00 p.m.<o:p></o:p></font></span></p><p class="MsoNormal" align="center" style="text-align:center;tab-stops:right 6.5in"><!--[if gte vml 1]><v:line
id="_x0000_s1026" style='position:absolute;left:0;text-align:left;z-index:251657728'
from="0,11.5pt" to="470.25pt,11.5pt" strokecolor="#969696" strokeweight="2.25pt"/><![endif]--><!--[if !vml]--><font class="Apple-style-span" size="4"><span style="mso-ignore:vglayout;position:relative;z-index:251657728"><span style="left:0px;position:absolute;left:-5px;top:-91px;width:477px;height:7px"><font class="Apple-style-span" face="Times"><br></font></span></span><!--[endif]--></font></p><font class="Apple-style-span" size="4"><br style="mso-ignore:vglayout" clear="ALL"></font><p class="MsoNormal" style="text-align:justify"><font class="Apple-style-span" size="4">Development of functional engineered
tissues has the potential to improve significantly current approaches aimed at
repairing or replacing damaged tissues and organs. In addition, engineered
tissues could serve as highly relevant and useful models for disease and for
screening novel drugs and therapies. Methods to assess the development and
function of these tissues are traditionally limited by their invasive and
destructive nature. Our recent work demonstrates that optical spectroscopic
imaging approaches can overcome these limitations and provide functional
biochemical and organizational/structural information about tissues. We
specifically aim to identify optical biomarkers associated with adipose and
bone engineered tissue development and early cancer development using
non-linear spectroscopic imaging methods that rely on endogenous fluorescence
and scattering. Intrinsic two photon excited fluorescence (TPEF) has been used
as the main platform to characterize tissue structure and function. Acquisition
of emission spectra at multiple excitation wavelengths reveals that key aspects
of the composition and organization of silk biomaterial scaffolds can be
assessed using TPEF and second harmonic generation (SHG). Similar spectroscopic
images also demonstrate that important biochemical and morphological parameters
associated with stem cell differentiation can be extracted in two and
three-dimensional cultures. Measurements performed on the same scaffolds over a
period of six months illustrate that significant dynamic biochemical changes
can be monitored to extract important insights in metabolic biochemical
pathways. Biochemical and subtle, microscale organizational differences can
also be detected as a function of depth within engineered normal and
pre-cancerous epithelial tissues. Such non-invasive biomarkers could be
ultimately used to develop improved diagnostics and to assess functional tissue
development either in response to different stimuli or as quality controls
prior to implantation. <o:p></o:p></font></p><p class="MsoNormal" align="center" style="text-align:center"><font class="Apple-style-span" size="4"><br></font></p><p class="MsoNormal" align="center" style="text-align:center"><font class="Apple-style-span" size="4">Grier Room, MIT Bldg 34-401<o:p></o:p></font></p><p class="MsoNormal" align="center" style="text-align:center"><font class="Apple-style-span" size="4">Refreshments served after the lecture</font><font class="Apple-style-span" size="5"><o:p></o:p></font></p>
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