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<div><font face="Times New Roman" size="+1" color="#000000"><br>
</font><font face="Arial" color="#000000">Seminar on</font></div>
<div><font face="Arial" color="#000000"><b>Modern Optics and
Spectroscopy<br>
<br>
Wonshik Choi</b>,<br>
Massachusetts Institute of Technology<br>
<br>
<i><b>Field-based 3D microscopy for live cell imaging<br>
<br>
</b></i>March 11, 2008</font><br>
<font face="Arial" color="#000000"></font></div>
<div><font face="Arial" color="#000000">12:00 - 1:00 p.m.</font></div>
<div><font face="Arial" color="#000000"><br>
</font><font face="Times New Roman" color="#000000">Over the past
decades powerful optical microscopy techniques for probing and imaging
biological cells and tissues have been developed. Advances have been
made to improve spatial and temporal resolution, chemical and
molecular specificities, and depth range of imaging. However, most of
these developments are based on measuring the intensity of light.
Our group has developed phase microscopy techniques for quantifying
not only the intensity but also the phase of the light, which we term
as a field-based technique in comparison with conventional
intensity-based technique. In this talk, we report a new field-based
3D microscope which can map the distribution of refractive index as
well as absorption in live cells and tissues. We demonstrate
tomographic imaging of cells and multicellular organisms, and
time-dependent changes in cell structure. Our results will enable
quantitative characterization of specimen-induced aberrations in
high-resolution microscopy, and have multiple applications in tissue
light scattering.</font></div>
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