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Hi all,<br>
<br>
This is my first post, sorry if it sounds too naive. I have read the
paper and the supplementary, but I still have some trouble
understanding the way sample_counts in (1,1) go to sample_assigned.
<br>
<br>
For example, for finding the PSI values for SE events in a
particular sample, I have the following outputs for three events:<br>
event=>
chr2:238427182:238427291:+@chr2:238428553:238428672:+@chr2:238434244:238434448:+<br>
counts => (0,0):1028,(0,1):10,(1,0):422,(1,1):462<br>
assigned_counts=> 0:756,1:138<br>
<b>334 (1,1) counts assigned to first isoform, 128 (1,1) counts
assigned to second isoform.</b><br>
<br>
event=>chr2:70520750:70520869:-@chr2:70516482:70516504:-@chr2:70515200:70515324:-<br>
counts=>(0,0):126,(0,1):4,(1,0):54,(1,1):186<br>
assigned_counts=>0:207,1:37<br>
<b>153 (1,1) counts assigned to first isoform, 33 (1,1) counts
assigned to second isoform.</b><br>
<br>
event=>chr2:122513236:122513529:+@chr2:122514816:122515010:+@chr2:122516286:122516382:+<br>
counts=>(0,0):46,(1,0):5,(1,1):27<br>
assigned_counts=>0:8,1:24<br>
<b>3 (1,1) counts assigned to first isoform, 24 (1,1) counts
assigned to second isoform. </b><br>
<br>
I realize there may be a large number of factors that may affect the
way (1,1) counts are assigned but I am a bit confused as to why for
the last event, why 24 (1,1) counts were assigned to second isoform
when there was no evidence for (0,1) counts in that event.<br>
<br>
Any help with insights with factors that affect the way (1,1) counts
are assigned would be most appreciated. Thank you in advance.<br>
<br>
Regards,<br>
<br>
<br>
J<br>
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