<html><body><div style="color:#000; background-color:#fff; font-family:times new roman, new york, times, serif;font-size:12pt"><div id="yiv8485414121"><div><div style="color: rgb(0, 0, 0); background-color: rgb(255, 255, 255); font-family: 'times new roman', 'new york', times, serif; font-size: 12pt;"><div id="yiv8485414121yui_3_7_2_31_1370426016331_39">Dear MISO users,</div><div id="yiv8485414121yui_3_7_2_31_1370426016331_39"><br></div><div id="yiv8485414121yui_3_7_2_31_1370426016331_39" style="color: rgb(0, 0, 0); font-size: 16px; font-family: 'times new roman', 'new york', times, serif; background-color: transparent; font-style: normal;">Warm Greetings !!</div><div id="yiv8485414121yui_3_7_2_31_1370426016331_39" style="color: rgb(0, 0, 0); font-size: 16px; font-family: 'times new roman', 'new york', times, serif; background-color: transparent; font-style: normal;"><br id="yiv8485414121yui_3_7_2_31_1370426016331_65"></div><div
id="yiv8485414121yui_3_7_2_31_1370426016331_39" style="color: rgb(0, 0, 0); font-size: 16px; font-family: 'times new roman', 'new york', times, serif; background-color: transparent; font-style: normal;">I am Sudeep from India. Recently I have been
working on alternative splicing. </div><div id="yiv8485414121yui_3_7_2_31_1370426016331_39" style="color: rgb(0, 0, 0); font-size: 16px; font-family: 'times new roman', 'new york', times, serif; background-color: transparent; font-style: normal;">My question is regarding extraction of genes which show more than two isoforms. </div><div id="yiv8485414121yui_3_7_2_31_1370426016331_39" style="color: rgb(0, 0, 0); font-size: 16px; font-family: 'times new roman', 'new york', times, serif; background-color: transparent; font-style: normal;"><br id="yiv8485414121yui_3_7_2_31_1370426016331_80"></div><div id="yiv8485414121yui_3_7_2_31_1370426016331_39" style="color: rgb(0, 0, 0); font-size: 16px; font-family: 'times new roman', 'new york', times, serif; background-color: transparent; font-style: normal;">Summary about my project: </div><div id="yiv8485414121yui_3_7_2_31_1370426016331_39" style="color: rgb(0, 0, 0); font-size: 16px; font-family:
'times new roman', 'new york', times, serif; background-color: transparent; font-style: normal;"><br id="yiv8485414121yui_3_7_2_31_1370426016331_90"></div><div id="yiv8485414121yui_3_7_2_31_1370426016331_39" style="color: rgb(0, 0, 0); font-size: 16px; font-family: 'times new roman', 'new york', times, serif; background-color: transparent; font-style: normal;">#1 Samples : 2 (case, control)</div><div id="yiv8485414121yui_3_7_2_31_1370426016331_39" style="color: rgb(0, 0, 0); font-size: 16px; font-family: 'times new roman', 'new york', times, serif; background-color: transparent; font-style: normal;">#2 Generated BAM files using Tophat.</div><div id="yiv8485414121yui_3_7_2_31_1370426016331_39" style="color: rgb(0, 0, 0); font-size: 16px; font-family: 'times new roman', 'new york', times, serif; background-color: transparent; font-style: normal;">#3 After comparison step, am stuck with filtering step. Error : More than two isoforms.</div><div
id="yiv8485414121yui_3_7_2_31_1370426016331_39" style="color: rgb(0, 0, 0); font-size: 16px; font-family: 'times new roman', 'new york', times, serif; background-color: transparent; font-style: normal;"><br id="yiv8485414121yui_3_7_2_31_1370426016331_101"></div><div id="yiv8485414121yui_3_7_2_31_1370426016331_39" style="color: rgb(0, 0, 0); font-size: 16px; font-family: 'times new roman', 'new york', times, serif; background-color: transparent; font-style: normal;">Please suggest me parameters values (parameters; --bayes-factor, --delta-psi) and any way to use these file to get filter genes and sashimi plot.</div><div id="yiv8485414121yui_3_7_2_31_1370426016331_39" style="color: rgb(0, 0, 0); font-size: 16px; font-family: 'times new roman', 'new york', times, serif; background-color: transparent; font-style: normal;"><br></div><div id="yiv8485414121yui_3_7_2_31_1370426016331_39" style="color:rgb(0, 0, 0);font-size:16px;font-family:'times new
roman', 'new york', times, serif;background-color:transparent;font-style:normal;" class="yui_3_7_2_31_1370427420742_67">Kind Regards,</div><div id="yiv8485414121yui_3_7_2_31_1370426016331_39" style="color:rgb(0, 0, 0);font-size:16px;font-family:'times new roman', 'new york', times, serif;background-color:transparent;font-style:normal;" class="yui_3_7_2_31_1370427420742_67">Sudeep</div></div></div></div></div></body></html>