[miso-users] new mouse genome annotation for alternative cleavage and polyadenylation events

Charizanis,Konstantinos quijotes at ufl.edu
Mon Jan 7 15:31:00 EST 2013


I used the new annotation (Bin Tian DEC 2012)  to do a previous analysis but I have the following problem. Miso works up to the comparing sample step. After that it gives an error when I try to filter the results.
I went and viewed the Bayes factors file output of the comparison and it doesn't make any sense. I'm copied the first couple rows.


event_name      sample1_posterior_mean  sample1_ci_low  sample1_ci_high sample2_posterior_mean  sample2_ci_low  sample2_ci_high diff    bayes_factor   etc.

Zyx:42307851    0.01,0.03,0.05,0.48,0.44        0.00,0.01,0.01,0.15,0.02        0.01,0.05,0.08,0.90,0.77        0.01,0.03,0.08,0.73,0.16        0.00,0.01,0.04,0.44,0.01        0.01,0.04,0.11,0.89,0.45    0.00,0.00,-0.03,-0.24,0.27      0.00,0.03,0.13,0.95,0.93        'Zyx:42307851.1.0','Zyx:42307851.2.0','Zyx:42307851.3.0','Zyx:42307851.4.0','Zyx:42307851.5.0'


I also checked the indexed gff annotation and it looks different from the previous annotations that exist on the MISO website.
Any input or ideas would be much appreciated.
Thank you

Konstantinos
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