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<p class="MsoNormal" style="mso-margin-top-alt:auto;mso-margin-bottom-alt:auto">Begin forwarded message:<o:p></o:p></p>
<p class="MsoNormal" style="mso-margin-top-alt:auto;mso-margin-bottom-alt:auto"><b><span style="font-family:"Helvetica Neue"">Subject: Looking for a UROP student</span></b><o:p></o:p></p>
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<p class="MsoNormal"><b><span style="font-family:"Helvetica Neue"">Date: </span></b><span style="font-family:"Helvetica Neue"">August 26, 2019 at 5:28:05 PM EDT</span><o:p></o:p></p>
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<p class="MsoNormal" style="mso-margin-top-alt:auto;mso-margin-bottom-alt:auto"><b>Term:
</b>Fall & Spring<o:p></o:p></p>
<p class="MsoNormal" style="mso-margin-top-alt:auto;mso-margin-bottom-alt:auto"> <b>UROP Department, Lab or Center:
</b>Whitehead Institute, Biology (Course 7)<o:p></o:p></p>
<p class="MsoNormal" style="mso-margin-top-alt:auto;mso-margin-bottom-alt:auto"> <b>MIT Faculty Supervisor Name</b>: David Sabatini<o:p></o:p></p>
<p class="MsoNormal" style="mso-margin-top-alt:auto;mso-margin-bottom-alt:auto"> <b>Project Title:
</b> Understanding how mTORC1 regulates the efflux of essential amino acids from the lysosome<o:p></o:p></p>
<p class="MsoNormal" style="mso-margin-top-alt:auto;mso-margin-bottom-alt:auto"> <b>Project Description:
</b> <o:p></o:p></p>
<p class="MsoNormal" style="mso-margin-top-alt:auto;mso-margin-bottom-alt:auto">The mechanistic target of rapamycin complex 1 (mTORC1) kinase is a crucial regulator of cell growth and metabolism. Recently, using a novel method developed in the lab to rapidly
purify lysosomes, we found mTORC1 regulates the lysosomal abundance of essential amino acids through a mechanism that involves the amino acid transporter SLC38A9. We are now working to understand how exactly mTORC1 regulates the transport function of SLC38A9.
Understanding this process may be of particular value in Ras-driven pancreatic cancers, which not only depend heavily on micropinocytosis to sustain their amino acid levels, and for cell survival and proliferation, but also
<span style="font-family:"Times New Roman",serif">require SLC38A9 to form tumors in vivo.</span><o:p></o:p></p>
<p class="MsoNormal" style="mso-margin-top-alt:auto;mso-margin-bottom-alt:auto">The Sabatini lab is looking for a student who is interested in cancer research, metabolism and biochemistry to assist with this project. This opportunity is offered for the Fall
and Spring terms with possibility for continuation during next summer. The student will learn a variety of techniques including mammalian cell culture, protein purification and various types of biochemical assays.<o:p></o:p></p>
<p class="MsoNormal" style="mso-margin-top-alt:auto;mso-margin-bottom-alt:auto">If interested, please send your CV/resume and detail any experience or course work you have had with molecular biology and experimental biology.<o:p></o:p></p>
<p class="MsoListParagraph" style="text-indent:-.25in"><span style="font-family:Symbol">·</span><span style="font-size:7.0pt;font-family:"Times New Roman",serif">
</span>Academic year 2019/20202<o:p></o:p></p>
<p class="MsoListParagraph" style="text-indent:-.25in"><span style="font-family:Symbol">·</span><span style="font-size:7.0pt;font-family:"Times New Roman",serif">
</span>Direct Supervisor: Jibril Kedir<o:p></o:p></p>
<p class="MsoNormal" style="mso-margin-top-alt:auto;mso-margin-bottom-alt:auto"> <o:p></o:p></p>
<p class="MsoNormal" style="mso-margin-top-alt:auto;mso-margin-bottom-alt:auto"><b>Prerequisites:</b>
<o:p></o:p></p>
<p class="MsoListParagraph" style="text-indent:-.25in"><span style="font-family:Symbol">·</span><span style="font-size:7.0pt;font-family:"Times New Roman",serif">
</span>Cell culture experience<o:p></o:p></p>
<p class="MsoListParagraph" style="text-indent:-.25in"><span style="font-family:Symbol">·</span><span style="font-size:7.0pt;font-family:"Times New Roman",serif">
</span>Good communication and organizational skills<o:p></o:p></p>
<p class="MsoListParagraph" style="text-indent:-.25in"><span style="font-family:Symbol">·</span><span style="font-size:7.0pt;font-family:"Times New Roman",serif">
</span>Background in experimental and molecular biology<o:p></o:p></p>
<p class="MsoListParagraph" style="text-indent:-.25in"><span style="font-family:Symbol">·</span><span style="font-size:7.0pt;font-family:"Times New Roman",serif">
</span>Interest in pursuing a graduate degree in the life sciences is considered a plus<o:p></o:p></p>
<p class="MsoNormal" style="mso-margin-top-alt:auto;mso-margin-bottom-alt:auto"> <o:p></o:p></p>
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