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<p class="xmsonormal">****<o:p></o:p></p>
<p class="xmsonormal">For more information on this posting please contact:<o:p></o:p></p>
<p class="xmsonormal">Grabowski, Eric F.,M.D.,ScD." <<a href="mailto:EGRABOWSKI@mgh.harvard.edu"><span style="color:purple">EGRABOWSKI@mgh.harvard.edu</span></a><o:p></o:p></p>
<p class="xmsonormal">The Cardiovascular Thrombosis Laboratory, located in the Jackson Building on the MGH main campus, is in need of a full-time person with experimental and analytical skills for studies of endothelial cell and blood platelet function. The
work (see responsibilities below) concerns the study of blood coagulation and thrombosis in
<i><u>in vitro</u></i> models of blood vessels, with applications to the childhood hemolytic uremic syndrome, adult arterial thrombosis, and bleeding disorders such as Von Willebrand Disease. The position provides ample opportunity to work at the forefront
of vascular biology, specifically in mRNA isolation, qPCR, the hemodynamics of flowing blood, fluorescence digital videomicroscopy, and digital image processing (e.g., two antigen co-localization in real-time) using state-of-the-art software (MetaMorph) and
hardware (z-motor, liquid light guide). Training in the use of this software will be available. While endothelial cell cultures will be used in experiments, so, too, will be an important genetically-modified mouse model that will be maintained by another
lab member. Importantly, the successful applicant will also enjoy working on problems of direct relevance to future patient care and even, in some instances, clinical studies which utilize our laboratory assays.<o:p></o:p></p>
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<p class="xmsonormal" style="margin-left:.25in">Responsibilities include, but are not limited to:<o:p></o:p></p>
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<li class="xmsonormal" style="mso-list:l0 level1 lfo1">Assisting in the maintenance of endothelial cell cultures,
<o:p></o:p></li><li class="xmsonormal" style="mso-list:l0 level1 lfo1">Assistance in the utilization of a unique, genetically modified mouse model
<o:p></o:p></li><li class="xmsonormal" style="mso-list:l0 level1 lfo1">Assisting the laboratory director and other lab personnel in research projects involving chromogenic assays for tissue factor activity,
<o:p></o:p></li><li class="xmsonormal" style="mso-list:l0 level1 lfo1">Performing digital image analysis of platelet-endothelial cell interactions in flowing blood,
<o:p></o:p></li><li class="xmsonormal" style="mso-list:l0 level1 lfo1">Assisting the laboratory director and others in mRNA isolation and qPCR,
<o:p></o:p></li><li class="xmsonormal" style="mso-list:l0 level1 lfo1">Collection of and accurate recording of procedures and results,
<o:p></o:p></li><li class="xmsonormal" style="mso-list:l0 level1 lfo1">Helping in the maintenance of databases used in the lab,
<o:p></o:p></li><li class="xmsonormal" style="mso-list:l0 level1 lfo1">Assistance in ordering and managing the use of necessary reagents,
<o:p></o:p></li><li class="xmsonormal" style="mso-list:l0 level1 lfo1">Participating in and presenting data at weekly lab meetings and journal clubs.
<o:p></o:p></li></ul>
<p class="xmsonormal">Experience with sterile cell culture techniques and with current approaches used in basic molecular biology and biochemistry are helpful. If a bioengineering graduate, knowledge of convective diffusion and principles of digital image analysis
are helpful. Most important, however, is an ability of the applicant to build upon his or her present knowledge bases, and to learn new techniques or to develop new ones as necessary to contribute to our research projects. Basic proficiency in Excel, Word,
Power point, and Internet searches is desirable.<o:p></o:p></p>
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<p class="xmsonormal">Duties will be performed in a research laboratory. Direct patient contact may occur in the process of assisting the laboratory director and other personnel in the collection and transport of blood specimens from the clinic to the laboratory.<o:p></o:p></p>
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<p class="MsoNormal"><b>From:<span class="xapple-converted-space"> </span></b>"Grabowski, Eric F.,M.D.,ScD." <<a href="mailto:EGRABOWSKI@mgh.harvard.edu"><span style="color:purple">EGRABOWSKI@mgh.harvard.edu</span></a>><o:p></o:p></p>
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<p class="MsoNormal"><b>Subject: Re: Research job at MGH</b><o:p></o:p></p>
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<p class="MsoNormal"><span style="font-size:10.5pt">Would you kindly post the attached job for your new graduates? This is in vascular biology research at MGH. </span><o:p></o:p></p>
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<p class="MsoNormal"><span style="font-size:10.5pt">With best regards,</span><o:p></o:p></p>
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<p class="MsoNormal"><span style="font-size:10.5pt"> </span><o:p></o:p></p>
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<p class="MsoNormal"><span style="font-size:10.5pt">Eric F. Grabowski, MD, DEngrSci</span><o:p></o:p></p>
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<p class="MsoNormal"><span style="font-size:10.5pt">Associate Professor of Pediatrics</span><o:p></o:p></p>
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<p class="MsoNormal"><span style="font-size:10.5pt">Director, Cardiovascular Thrombosis Laboratory</span><o:p></o:p></p>
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<p class="MsoNormal"><span style="font-size:10.5pt">Massachusetts General Hospital</span><o:p></o:p></p>
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<p class="MsoNormal"><span style="font-size:10.5pt">Harvard Medical School</span><o:p></o:p></p>
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<p class="MsoNormal"><span style="font-size:10.5pt">617-726-2737</span><o:p></o:p></p>
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<p class="MsoNormal"><span style="font-size:10.5pt">FAX: 617-724-0702</span><o:p></o:p></p>
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