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<p class="MsoNormal"><a name="_MailOriginalBody">Begin forwarded message:<o:p></o:p></a></p>
<p class="MsoNormal"><span style="mso-bookmark:_MailOriginalBody"><o:p> </o:p></span></p>
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<p class="MsoNormal"><span style="mso-bookmark:_MailOriginalBody"><b><span style="font-family:"Helvetica Neue"">From:
</span></b></span><span style="mso-bookmark:_MailOriginalBody"><span style="font-family:"Helvetica Neue"">Emily Hueske <</span></span><a href="mailto:ehanna@mit.edu"><span style="mso-bookmark:_MailOriginalBody"><span style="font-family:"Helvetica Neue"">ehanna@mit.edu</span></span><span style="mso-bookmark:_MailOriginalBody"></span></a><span style="mso-bookmark:_MailOriginalBody"><span style="font-family:"Helvetica Neue"">></span></span><span style="mso-bookmark:_MailOriginalBody"><o:p></o:p></span></p>
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<p class="MsoNormal"><span style="mso-bookmark:_MailOriginalBody"><b><span style="font-family:"Helvetica Neue"">Subject: UROP: Develop molecular genetic approaches targeting genes of interest in brains of mouse models of neurodegenerative disease</span></b></span><span style="mso-bookmark:_MailOriginalBody"><o:p></o:p></span></p>
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<p class="MsoNormal"><span style="mso-bookmark:_MailOriginalBody"><b><span style="font-family:"Helvetica Neue"">Date:
</span></b></span><span style="mso-bookmark:_MailOriginalBody"><span style="font-family:"Helvetica Neue"">February 5, 2018 at 3:40:56 PM EST</span></span><span style="mso-bookmark:_MailOriginalBody"><o:p></o:p></span></p>
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<p class="MsoNormal"><span style="mso-bookmark:_MailOriginalBody"><b><span style="font-family:"Helvetica Neue"">Cc:
</span></b></span><span style="mso-bookmark:_MailOriginalBody"><span style="font-family:"Helvetica Neue"">Alexander Friedman <</span></span><a href="mailto:afried@mit.edu"><span style="mso-bookmark:_MailOriginalBody"><span style="font-family:"Helvetica Neue"">afried@mit.edu</span></span><span style="mso-bookmark:_MailOriginalBody"></span></a><span style="mso-bookmark:_MailOriginalBody"><span style="font-family:"Helvetica Neue"">></span></span><span style="mso-bookmark:_MailOriginalBody"><o:p></o:p></span></p>
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<p class="MsoNormal"><span style="mso-bookmark:_MailOriginalBody"><o:p> </o:p></span></p>
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<p class="MsoNormal" style="background:white"><span style="mso-bookmark:_MailOriginalBody"><span style="font-size:9.5pt;font-family:"Arial",sans-serif;color:#222222">Hi there, <o:p></o:p></span></span></p>
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<p class="MsoNormal" style="background:white"><span style="mso-bookmark:_MailOriginalBody"><span style="font-size:9.5pt;font-family:"Arial",sans-serif;color:#222222"><o:p> </o:p></span></span></p>
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<p class="MsoNormal" style="background:white"><span style="mso-bookmark:_MailOriginalBody"><span style="font-size:9.5pt;font-family:"Arial",sans-serif;color:#222222">Would it be possible to send the following UROP posting to biology and bioengineering undergraduate
students? <o:p></o:p></span></span></p>
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<p class="MsoNormal" style="background:white"><span style="mso-bookmark:_MailOriginalBody"><span style="font-size:9.5pt;font-family:"Arial",sans-serif;color:#222222"><o:p> </o:p></span></span></p>
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<p class="MsoNormal" style="background:white"><span style="mso-bookmark:_MailOriginalBody"><span style="font-size:9.5pt;font-family:"Arial",sans-serif;color:#222222">Thank you and best regards, <o:p></o:p></span></span></p>
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<p class="MsoNormal" style="background:white"><span style="mso-bookmark:_MailOriginalBody"><span style="font-size:9.5pt;font-family:"Arial",sans-serif;color:#222222">Emily and Alexander<o:p></o:p></span></span></p>
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<p class="MsoNormal" style="background:white"><span style="mso-bookmark:_MailOriginalBody"><span style="font-size:9.5pt;font-family:"Arial",sans-serif;color:#222222"><o:p> </o:p></span></span></p>
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<p class="MsoNormal" style="background:white"><span style="mso-bookmark:_MailOriginalBody"><span style="font-size:10.0pt;font-family:"Arial",sans-serif;color:#222222">Emily Hueske, PhD</span></span><span style="mso-bookmark:_MailOriginalBody"><span style="font-size:9.5pt;font-family:"Arial",sans-serif;color:#222222"><o:p></o:p></span></span></p>
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<p class="MsoNormal" style="background:white"><span style="mso-bookmark:_MailOriginalBody"><span style="font-size:10.0pt;font-family:"Arial",sans-serif;color:#222222">Research Scientist</span></span><span style="mso-bookmark:_MailOriginalBody"><span style="font-size:9.5pt;font-family:"Arial",sans-serif;color:#222222"><o:p></o:p></span></span></p>
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<p class="MsoNormal" style="background:white"><span style="mso-bookmark:_MailOriginalBody"><span style="font-size:10.0pt;font-family:"Arial",sans-serif;color:#222222">Graybiel Laboratory</span></span><span style="mso-bookmark:_MailOriginalBody"><span style="font-size:9.5pt;font-family:"Arial",sans-serif;color:#222222"><o:p></o:p></span></span></p>
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<p class="MsoNormal" style="background:white"><span style="mso-bookmark:_MailOriginalBody"><span style="font-size:10.0pt;font-family:"Arial",sans-serif;color:#222222">McGovern Institute for Brain Research</span></span><span style="mso-bookmark:_MailOriginalBody"><span style="font-size:9.5pt;font-family:"Arial",sans-serif;color:#222222"><o:p></o:p></span></span></p>
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<p class="MsoNormal" style="background:white"><span style="mso-bookmark:_MailOriginalBody"><span style="font-size:10.0pt;font-family:"Arial",sans-serif;color:#222222">Massachusetts Institute of Technology</span></span><span style="mso-bookmark:_MailOriginalBody"><span style="font-size:9.5pt;font-family:"Arial",sans-serif;color:#222222"><o:p></o:p></span></span></p>
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<p class="MsoNormal" style="background:white"><span style="mso-bookmark:_MailOriginalBody"><span style="font-size:10.0pt;font-family:"Arial",sans-serif;color:#222222">77 Massachusetts Ave, Cambridge MA 02139</span></span><span style="mso-bookmark:_MailOriginalBody"><span style="font-size:9.5pt;font-family:"Arial",sans-serif;color:#222222"><o:p></o:p></span></span></p>
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<p class="MsoNormal" style="background:white"><span style="mso-bookmark:_MailOriginalBody"><span style="font-size:9.5pt;font-family:"Arial",sans-serif;color:#222222"><o:p> </o:p></span></span></p>
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<p class="MsoNormal" style="background:white"><span style="mso-bookmark:_MailOriginalBody"><span style="font-size:9.5pt;font-family:"Arial",sans-serif;color:#222222"><o:p> </o:p></span></span></p>
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<p class="MsoNormal" style="background:white"><span style="mso-bookmark:_MailOriginalBody"><span style="font-size:9.5pt;font-family:"Arial",sans-serif;color:#222222">Department/Lab: McGovern Institute for Brain Research <o:p></o:p></span></span></p>
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<p class="MsoNormal" style="background:white"><span style="mso-bookmark:_MailOriginalBody"><span style="font-size:9.5pt;font-family:"Arial",sans-serif;color:#222222">Faculty Supervisor: Ann M Graybiel <o:p></o:p></span></span></p>
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<p class="MsoNormal" style="background:white"><span style="mso-bookmark:_MailOriginalBody"><span style="font-size:9.5pt;font-family:"Arial",sans-serif;color:#222222">Emily Hueske <o:p></o:p></span></span></p>
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<p class="MsoNormal" style="background:white"><span style="mso-bookmark:_MailOriginalBody"><span style="font-size:9.5pt;font-family:"Arial",sans-serif;color:#222222"><o:p> </o:p></span></span></p>
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<p class="MsoNormal" style="background:white"><span style="mso-bookmark:_MailOriginalBody"><span style="font-size:9.5pt;font-family:"Arial",sans-serif;color:#222222"><o:p> </o:p></span></span></p>
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<p class="MsoNormal" style="background:white"><span style="mso-bookmark:_MailOriginalBody"><span style="font-size:9.5pt;font-family:"Arial",sans-serif;color:#222222">Project Title: Develop molecular genetic and <span class="gmail-il">CRISPR</span> constructs
for targeting genes of interest in brains of mouse models of neurodegenerative disease <o:p></o:p></span></span></p>
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<p class="MsoNormal" style="background:white"><span style="mso-bookmark:_MailOriginalBody"><span style="font-size:9.5pt;font-family:"Arial",sans-serif;color:#222222"><o:p> </o:p></span></span></p>
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<p class="MsoNormal" style="background:white"><span style="mso-bookmark:_MailOriginalBody"><span style="font-size:9.5pt;font-family:"Arial",sans-serif;color:#222222"><o:p> </o:p></span></span></p>
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<p class="MsoNormal" style="background:white"><span style="mso-bookmark:_MailOriginalBody"><span style="font-size:9.5pt;font-family:"Arial",sans-serif;color:#222222">Project Description: Help us solve the mysteries of the brain using your molecular cloning
skills! <o:p></o:p></span></span></p>
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<p class="MsoNormal" style="background:white"><span style="mso-bookmark:_MailOriginalBody"><span style="font-size:9.5pt;font-family:"Arial",sans-serif;color:#222222"><o:p> </o:p></span></span></p>
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<p class="MsoNormal" style="background:white"><span style="mso-bookmark:_MailOriginalBody"><span style="font-size:9.5pt;font-family:"Arial",sans-serif;color:#222222">This is an ideal project for students interested in putting their molecular biology experience
to work toward unraveling the brain mechanisms of neurodegenerative disease, particularly Huntington's disease. <o:p></o:p></span></span></p>
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<p class="MsoNormal" style="background:white"><span style="mso-bookmark:_MailOriginalBody"><span style="font-size:9.5pt;font-family:"Arial",sans-serif;color:#222222"><o:p> </o:p></span></span></p>
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<p class="MsoNormal" style="background:white"><span style="mso-bookmark:_MailOriginalBody"><span style="font-size:9.5pt;font-family:"Arial",sans-serif;color:#222222">You will help us to develop and clone new constructs to target genes in the basal ganglia system
of mice. We will design and clone constructs for packaging in viral vectors. These vectors will be delivered to brain systems, in particularly the striatum and dopaminergic systems in mice and evaluated by anatomical/histological, and behavioral evaluations.
This project combines molecular biology skills, surgical skills, anatomical and behavioral genetic approaches. The student will be fully supervised and can work on a flexible time schedule. The ideal applicant for this <span class="gmail-il">UROP</span> position
will have experience with molecular cloning; prior experience with rodent work at MIT is a plus.<o:p></o:p></span></span></p>
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<p class="MsoNormal" style="background:white"><span style="mso-bookmark:_MailOriginalBody"><span style="font-size:9.5pt;font-family:"Arial",sans-serif;color:#222222"><o:p> </o:p></span></span></p>
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<p class="MsoNormal" style="background:white"><span style="mso-bookmark:_MailOriginalBody"><span style="font-size:9.5pt;font-family:"Arial",sans-serif;color:#222222">Ideally, we would like to find a <span class="gmail-il">UROP</span> who could work at least
12 hours per week and would potentially be interested in working with us for longer than a year. <o:p></o:p></span></span></p>
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<p class="MsoNormal" style="background:white"><span style="mso-bookmark:_MailOriginalBody"><span style="font-size:9.5pt;font-family:"Arial",sans-serif;color:#222222"><o:p> </o:p></span></span></p>
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<p class="MsoNormal" style="background:white"><span style="mso-bookmark:_MailOriginalBody"><span style="font-size:9.5pt;font-family:"Arial",sans-serif;color:#222222">In this project, our goal is to understand the functions of neurons in the striatum and other
brain areas in simple behavioral tasks performed by rats and mice, typically involving learning. The striatum is a key part of the basal ganglia that receives input from midbrain dopamine neurons, cortex, and thalamus. It is thought to be centrally involved
in procedural learning, habit formation, action selection, and movement disorders like Parkinson’s disease, Huntington’s disease, and dystonia, as well as addiction, depression, obsessive-compulsive disorder, Tourette syndrome, schizophrenia, and other disorders.<o:p></o:p></span></span></p>
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<p class="MsoNormal" style="background:white"><span style="mso-bookmark:_MailOriginalBody"><span style="font-size:9.5pt;font-family:"Arial",sans-serif;color:#222222"><o:p> </o:p></span></span></p>
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<p class="MsoNormal" style="background:white"><span style="mso-bookmark:_MailOriginalBody"><span style="font-size:9.5pt;font-family:"Arial",sans-serif;color:#222222">Prerequisites: Ideally, we would like to find a <span class="gmail-il">UROP</span> who can
work at least 12 hours per week. <span class="gmail-il">UROP</span> projects are for credit; with increasing experience or in special cases, we will consider <span class="gmail-il">UROPs</span> for pay.<o:p></o:p></span></span></p>
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<p class="MsoNormal" style="background:white"><span style="mso-bookmark:_MailOriginalBody"><span style="font-size:9.5pt;font-family:"Arial",sans-serif;color:#222222"><o:p> </o:p></span></span></p>
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<p class="MsoNormal" style="background:white"><span style="mso-bookmark:_MailOriginalBody"><span style="font-size:9.5pt;font-family:"Arial",sans-serif;color:#222222">Contact: Emily Hueske (</span></span><a href="mailto:ehanna@mit.edu" target="_blank"><span style="mso-bookmark:_MailOriginalBody"><span style="font-size:9.5pt;font-family:"Arial",sans-serif;color:#1155CC">ehanna@mit.edu</span></span><span style="mso-bookmark:_MailOriginalBody"></span></a><span style="mso-bookmark:_MailOriginalBody"><span style="font-size:9.5pt;font-family:"Arial",sans-serif;color:#222222">)
and Alexander Friedman, PhD (</span></span><a href="mailto:afried@mit.edu" target="_blank"><span style="mso-bookmark:_MailOriginalBody"><span style="font-size:9.5pt;font-family:"Arial",sans-serif;color:#1155CC">afried@mit.edu</span></span><span style="mso-bookmark:_MailOriginalBody"></span></a><span style="mso-bookmark:_MailOriginalBody"><span style="font-size:9.5pt;font-family:"Arial",sans-serif;color:#222222">)<o:p></o:p></span></span></p>
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