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<div class="gmail_quote">_____________________________<br>
From: Jared Kehe <<a dir="ltr" href="mailto:jkehe@broadinstitute.org" x-apple-data-detectors="true" x-apple-data-detectors-type="link" x-apple-data-detectors-result="0">jkehe@broadinstitute.org</a>><br>
Subject: MIT Microbiome Club - Seminar Series - Dr. Cullen R. Buie<br>
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<font class="" color="#89a65e" size="5" face="Avenir-Book">MIT Microbiome Club </font></div>
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<font class="" size="5" color="#89a65e" face="Avenir-Book">Seminar Series</font></div>
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<font class="" size="5" color="#2ea07e" face="Avenir-Book"><img id="D157D089-CE96-401B-B492-26D9537EC013" height="146" width="150" src="cid:DE188664-4E8D-43E8-AA08-7505B7991C01@broadinstitute.org" class=""></font></div>
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<font class="" color="#2ea07e" face="Avenir-Book"><span class=""><span class="" style="font-size: 40px;"><span class="" style="text-align: start;">Dr. Cullen R. Buie:</span></span></span></font></div>
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<font class="" color="#2ea07e" face="Avenir-Book"><span class=""><span class="" style="font-size: 40px;"><span class="" style="text-align: start;">Shining Light on Microbial Dark Matter with Microfluidics</span></span></span></font></div>
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<span class=""><font size="4" class="" color="#4a4f56" face="Avenir-Book">Friday, April 28 from 3–4pm in MIT 16-220</font></span></div>
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<a href="https://www.facebook.com/events/1838777896381706/" class=""><font class="" face="Avenir-Book"><span class="" style="font-size: 20px;">PLEASE RSVP ON OUR EVENT PAGE</span></font></a></div>
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<div class="" style="line-height: 1.38; margin-top: 0pt; margin-bottom: 0pt;"><font class="" face="Avenir-Book" style="font-size: 14px; color: rgb(75, 79, 86);">The MIT Microbiome Club will be hosting <a href="http://web.mit.edu/lemi/pers_faculty.html" class="">Dr.
Cullen R. Buie</a>.</font></div>
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Genetic sequencing reveals abundant microbial diversity in nature with an estimated number of 2X106 prokaryotic species in the sea and 4X106 in soil. However, the number of cultivated species listed in DSMZ, one of the largest biological resource centers worldwide,
is only on the order 15,000. Thus the majority of species on the planet can be classified as microbial dark matter with unknown and unexplored functionality. It is believed that one reason many species have evaded cultivation is that they rely upon syntrophic
interactions within their community, yet traditional microbiological techniques focus on species isolation. To increase the pool of cultivated species, we have developed nanoporous microscale microbial incubators (NMMI), systems that enable high-throughput
screening and real-time observation of multi-species co-culture. The key innovation in NMMI is that it enables physical isolation of microbes while facilitating chemical communication. As a proof of concept, a pair of quorum sensing (QS) cells is constructed
from wild type Escherichia coli K12 to test the NMMI device. The sender cell is transformed to constitutively express red fluorescent protein (RFP) and encode QS molecules (N-Acyl homoserine lactone, AHL) upon high cell concentration. The receiver cell is
transformed to express green fluorescent protein (GFP) upon receiving the AHL molecule. We seed the NMMI stochastically such that there is initially one cell per well on average. After several hours of cultivation we observe physically segregated green and
red fluorescent wells, confirming that AHL molecules have diffused from the sender cells to the receiver cells to activate GFP expression in the receiver. This platform enables high throughput cell culture and real-time observation of bacterial community growth
dynamics and could facilitate the discovery of previously uncultivated microbes.
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organisms that can be cultivated, it is well known that most are not amenable to genetic transformation, a foundational tool for both metabolic engineering and synthetic biology. One of the most robust forms of genetic transformation is electroporation in
which high electric fields (order 10 kV/cm) disrupt the cell envelope to introduce foreign nucleic acids. We have developed a rapid microfluidic assay to quantitatively measure the electric field conditions required to open pores in bacteria using electroporation.
Our rapid microfluidic electroporation assay can evaluate a range of electroporation conditions in a fraction of a second, a process that previously took hours or even days. Further, we have recently devised a microfluidic platform for high throughput electroporation
that can enable genetic transformation 103 104 times faster than conventional systems. Ultimately, processes that take over a year with conventional electroporation systems could be completed within a single day. Results of this work will broaden the scope
of bacteria available for applications of synthetic biology ranging from biofuel production to human microbiome based therapeutics.</span> </span></span></font><a href="https://www.facebook.com/events/1838777896381706/" class="">Learn more on the event page.</a><br class="">
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<div class="" style="line-height: 1.38; margin-top: 0pt; margin-bottom: 0pt;"><font face="Avenir-Book" class="">———</font></div>
<div class="" style="line-height: 1.38; margin-top: 0pt; margin-bottom: 0pt;"><font face="Avenir-Book" class=""><i class=""><span class="" style="background-color: rgb(255, 255, 255);"><font color="#1d2129" class="">The MIT Microbiome Club, part of the Center
for Microbiome Informatics and Therapeutics, </font></span><span class="" style="color: rgb(29, 33, 41); background-color: rgb(255, 255, 255);">brings together inquisitive MIT undergrads, grad researchers, postdocs, faculty, and clinicians in the emerging
field of microbiome studies and microbiome-based medicine. </span></i><a href="https://www.facebook.com/pg/MITmicrobiomeclub/about/?ref=page_internal" class="">Learn more on our Facebook page.</a></font></div>
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<div class=""><font face="Avenir-Book" class="">–</font></div>
<div class=""><font face="Avenir-Book" class="">Jared Kehe</font></div>
<div class=""><font face="Avenir-Book" class="">NSF Graduate Student, MIT Biological Engineering</font></div>
<div class=""><font face="Avenir-Book" class="">Blainey and Sabeti Labs, Broad Institute</font></div>
<div class=""><font face="Avenir-Book" class="">Broad Research Comm Lab Fellow</font></div>
<div class=""><font face="Avenir-Book" class=""><a href="mailto:jkehe@broadinstitute.org" class="">jkehe@broadinstitute.org</a></font></div>
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