<html>
<head>
<meta http-equiv="Content-Type" content="text/html; charset=iso-8859-1">
<base href="x-msg://154/">
</head>
<body style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space; ">
<div>
<div ocsi="0" fpstyle="1" style="font-family: Helvetica; font-size: medium; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; orphans: 2; text-align: -webkit-auto; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px; ">
<div style="direction: ltr; font-family: Tahoma; font-size: 10pt; ">
<div style="margin: 0px; "><font size="3" face="Times New Roman,serif"><span style="font-size: 12pt; "><font face="Cambria,serif">The faculty for 10.28, the Chemical-Biological Engineering Lab course, are looking for a few lab assistant for 10.28. A job
description is posted below. Please contact Dr. Hamel (<a href="mailto:jhamel@mit.edu">jhamel@mit.edu</a>) if you are interested.</font></span></font></div>
<div style="margin: 0px; "><font size="3" face="Times New Roman,serif"><span style="font-size: 12pt; "><font face="Cambria,serif"> </font></span></font></div>
<div style="margin: 0px; "><font size="3" face="Times New Roman,serif"><span style="font-size: 12pt; "><br>
</span></font></div>
<font size="3" face="Times New Roman,serif"><span style="font-size: 12pt; "><font face="Cambria,serif">Lab Assistant-Grader for 10.28 (10 hrs per week)</font></span></font>
<div>
<div style="margin: 0px; "><font size="3" face="Times New Roman,serif"><span style="font-size: 12pt; "><font face="Cambria,serif"> </font></span></font></div>
</div>
<div>
<div style="margin: 0px; "><font size="3" face="Times New Roman,serif"><span style="font-size: 12pt; "><font face="Cambria,serif">Requirements:</font></span></font></div>
</div>
<div>
<div style="margin: 0px; "><font size="3" face="Times New Roman,serif"><span style="font-size: 12pt; "><font face="Cambria,serif">Should be comfortable with handling chemicals and glassware.<br>
<br>
</font></span></font></div>
</div>
<div></div>
<div>
<div style="margin: 0px; "><font size="3" face="Times New Roman,serif"><span style="font-size: 12pt; "><font face="Cambria,serif">List of Responsibilities</font></span></font></div>
</div>
<div>
<div style="margin: 0px; "><font size="3" face="Times New Roman,serif"><span style="font-size: 12pt; "><font face="Cambria,serif">- Assisting with setting up equipment and materials for lab sessions each week</font></span></font></div>
</div>
<div>
<div style="margin: 0px; "><font size="3" face="Times New Roman,serif"><span style="font-size: 12pt; "><font face="Cambria,serif">- assembling bioreactors</font></span></font></div>
</div>
<div>
<div style="margin: 0px; "><font size="3" face="Times New Roman,serif"><span style="font-size: 12pt; "><font face="Cambria,serif">- autoclaving reagents and supplies</font></span></font></div>
</div>
<div>
<div style="margin: 0px; "><font size="3" face="Times New Roman,serif"><span style="font-size: 12pt; "><font face="Cambria,serif">- help with lab maintenance and safety, including leaning glassware and disposing of waste material</font></span></font></div>
</div>
<div style="margin: 0px; "><font size="3" face="Times New Roman,serif"><span style="font-size: 12pt; "><font face="Cambria,serif"> </font></span></font></div>
<div>
<div style="margin: 0px; "><font size="3" face="Times New Roman,serif"><span style="font-size: 12pt; "><font face="Cambria,serif">The position would offer an excellent opportunity to familiarize students with state-of-the-art bioreactor technology and bioprocessing.<br>
</font></span></font></div>
</div>
<br>
<br>
<br>
<div style="font-family: 'Times New Roman'; font-size: 16px; ">
<hr tabindex="-1">
<div id="divRpF742130" style="direction: ltr; "><font size="2" face="Tahoma"><b>From:</b><span class="Apple-converted-space"> </span>Joyce Roberge<br>
<b>Sent:</b><span class="Apple-converted-space"> </span>Tuesday, August 26, 2014 1:19 PM<br>
<b>To:</b><span class="Apple-converted-space"> </span><a href="mailto:bioundgrd@mit.edu">bioundgrd@mit.edu</a><br>
<b>Cc:</b><span class="Apple-converted-space"> </span>Jean-Francois P Hamel<br>
<b>Subject:</b><span class="Apple-converted-space"> </span>UROP Opportunity with Jean-Francois Hamel<br>
</font><br>
</div>
<div></div>
<div>
<div style="word-wrap: break-word; ">
<p class="x_MsoNormal" style="margin-top: 0px; margin-bottom: 0px; "></p>
</div>
<div style="word-wrap: break-word; ">
<p class="x_MsoNormal" style="margin-top: 0px; margin-bottom: 0px; "></p>
<div style="margin-top: 0px; margin-bottom: 0px; "><b>U</b><b>ROP Supervisor: Jean-Francois Hamel, PhD (</b><a href="mailto:jhamel@mit.edu" target="_blank"><b>jhamel@mit.edu</b></a><b>)</b></div>
<div style="margin-top: 0px; margin-bottom: 0px; "><b>Term: Fall 2014</b></div>
<div style="margin-top: 0px; margin-bottom: 0px; "><b>8/26/14</b></div>
<div style="margin-top: 0px; margin-bottom: 0px; "><b> </b></div>
<div style="margin-top: 0px; margin-bottom: 0px; text-align: center; line-height: 32px; ">
<b>Use of Ammonia Oxidizing Bacterium for Skin Therapy</b></div>
<div style="margin-top: 0px; margin-bottom: 0px; line-height: 32px; "><b><u><span style="font-family: 'Times New Roman'; ">Project Overview</span></u></b></div>
<div style="margin-top: 0px; margin-bottom: 0px; line-height: 32px; "><b>Background </b></div>
<div style="margin-top: 0px; margin-bottom: 0px; line-height: 32px; "><span style="font-family: 'Times New Roman'; "> Over the past decade, there has been a spike of interest in the human microbiome, which is the ecological community of commensal,
symbiotic, and pathogenic microorganisms that literally share our body space [1]. The importance of the human skin microbiome in skin health and a robust immune system has become a topic of increased interest. An important group of microorganisms, now virtually
absent from the skin of most humans, are ammonia oxidizing bacteria (AOB), bacteria ubiquitous in soils and untreated water [2]. AOB feed on the ammonia in sweat and convert it into nitrite and nitric oxide.</span></div>
<div style="margin-top: 0px; margin-bottom: 0px; text-indent: 0.5in; line-height: 32px; ">
<span style="font-family: 'Times New Roman'; ">Nitric oxide (NO) is a signaling mediator with many diverse biological activities. In the last few years, the importance of steady state NO concentrations has emerged as a key determinant of its biological function
[3]. Precise cellular responses are differentially regulated by specific NO concentration. Studies have correlated links between NO concentration and the regulation of synaptic transmission, blood pressure, vascular tone, immune cell function, and inflammation,
all of which are pertinent to human health [2, 4]. </span></div>
<div style="margin-top: 0px; margin-bottom: 0px; line-height: 32px; "><b>Introduction</b></div>
<div style="margin-top: 0px; margin-bottom: 0px; line-height: 32px; "><b><span style="font-family: 'Times New Roman'; "> <span class="Apple-converted-space"> </span></span></b><span style="font-family: 'Times New Roman'; ">Modern hygiene has selectively
depleted the natural balance of the skin microbiome particularly altering AOB. By restoring the appropriate AOB levels, scientists are working to impact a range of human health conditions.<span class="Apple-converted-space"> </span><i>Nitrosomonas eutropha</i>,
a common AOB, is currently being used by scientists to develop innovative bacterial therapies to explore the potential physiological beneficial effects of improving skin architecture, preventing infection, and improving vascularization [5].</span></div>
<div style="margin-top: 0px; margin-bottom: 0px; line-height: 32px; "><span style="font-family: 'Times New Roman'; "> Scientists have developed an innocuous solution containing<span class="Apple-converted-space"> </span><i>N. eutropha</i><span class="Apple-converted-space"> </span>as
a bacterial therapy skin care product. Early research supports that this low-concentration AOB treatment has positive effects on some hygiene factors such as odor and skin texture. Scientists are now looking test more concentrated forms of AOB for the treatment
of diabetic ulcers and other dermatologic conditions, and to scale up the production of the solution in order for the product to eventually reach the market.</span></div>
<div style="margin-top: 0px; margin-bottom: 0px; line-height: 32px; "><span style="font-family: 'Times New Roman'; "> The project would greatly benefit to produce cell at high concentrations. However it is currently not possible to do so, and we
have hypothesized that there is a metabolic bottleneck.</span></div>
<div style="margin-top: 0px; margin-bottom: 0px; line-height: 32px; "><b><u><span style="font-family: 'Times New Roman'; ">Responsibilities of the UROP student</span></u></b></div>
<div style="margin-top: 0px; margin-bottom: 0px; line-height: 32px; "><span style="font-family: 'Times New Roman'; "> The student will cultivate<span class="Apple-converted-space"> </span><i>N. eutropha</i><span class="Apple-converted-space"> </span>bacterium
in a state-of-the-art laboratory bioreactor, and measure cell growth and metabolic parameters. One specific feature of the bioreactor is that it is operated in the continuous mode. Prior knowledge of biological pathways and biochemistry would be helpful to
engage into the elucidation of the limited growth problem and to propose solutions.</span></div>
<div style="margin-top: 0px; margin-bottom: 0px; line-height: 32px; "><b> </b></div>
<div style="margin-top: 0px; margin-bottom: 0px; line-height: 32px; "><b><u><span style="font-family: 'Times New Roman'; ">References</span></u></b><b><span style="font-family: 'Times New Roman'; "></span></b></div>
<div style="margin-top: 0px; margin-bottom: 0px; text-indent: -0.25in; line-height: 24px; ">
<span style="font-family: 'Times New Roman'; ">1.<span style="font-size: 7pt; line-height: normal; "> <span class="Apple-converted-space"> </span></span></span><a href="http://www.nature.com/jid/journal/v131/n10/full/jid2011168a.html" target="_blank"><span style="font-family: 'Times New Roman'; ">http://www.nature.com/jid/journal/v131/n10/full/jid2011168a.html</span></a><span style="font-family: 'Times New Roman'; "></span></div>
<div style="margin-top: 0px; margin-bottom: 0px; text-indent: -0.25in; line-height: 24px; ">
<span style="font-family: 'Times New Roman'; ">2.<span style="font-size: 7pt; line-height: normal; "> <span class="Apple-converted-space"> </span></span></span><a href="http://download.springer.com/static/pdf/677/chp%253A10.1007%252F978-3-7643-8903-1_6.pdf?auth66=1403117403_98198d022fd2eea2d5bf0d19f684db0f&ext=.pdf" target="_blank"><span style="font-family: 'Times New Roman'; ">http://download.springer.com/static/pdf/677/chp%253A10.1007%252F978-3-7643-8903-1_6.pdf?auth66=1403117403_98198d022fd2eea2d5bf0d19f684db0f&ext=.pdf</span></a><span style="font-family: 'Times New Roman'; "></span></div>
<div style="margin-top: 0px; margin-bottom: 0px; text-indent: -0.25in; line-height: 24px; ">
<span style="font-family: 'Times New Roman'; ">3.<span style="font-size: 7pt; line-height: normal; "> <span class="Apple-converted-space"> </span></span></span><a href="http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2572721/" target="_blank"><span style="font-family: 'Times New Roman'; ">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2572721/</span></a><span style="font-family: 'Times New Roman'; "></span></div>
<div style="margin-top: 0px; margin-bottom: 0px; text-indent: -0.25in; line-height: 24px; ">
<span style="font-family: 'Times New Roman'; ">4.<span style="font-size: 7pt; line-height: normal; "> <span class="Apple-converted-space"> </span></span></span><a href="http://eurheartj.oxfordjournals.org/content/33/7/829.long" target="_blank"><span style="font-family: 'Times New Roman'; ">http://eurheartj.oxfordjournals.org/content/33/7/829.long</span></a><span style="font-family: 'Times New Roman'; "></span></div>
<div style="margin-top: 0px; margin-bottom: 0px; text-indent: -0.25in; line-height: 24px; ">
<span style="font-family: 'Times New Roman'; ">5.<span style="font-size: 7pt; line-height: normal; "> <span class="Apple-converted-space"> </span></span></span><a href="https://www.aobiome.com/science" target="_blank"><span style="font-family: 'Times New Roman'; ">https://www.aobiome.com/science</span></a><span style="font-family: 'Times New Roman'; "></span></div>
<div><span class="x_Apple-style-span" style="border-collapse: separate; border-spacing: 0px; ">
<div><br>
</div>
</span><br class="x_Apple-interchange-newline">
</div>
<br>
</div>
</div>
</div>
</div>
</div>
<br class="Apple-interchange-newline">
</div>
<div apple-content-edited="true"><span class="Apple-style-span" style="border-collapse: separate; color: rgb(0, 0, 0); font-family: Helvetica; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; orphans: 2; text-align: -webkit-auto; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-border-horizontal-spacing: 0px; -webkit-border-vertical-spacing: 0px; -webkit-text-decorations-in-effect: none; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px; font-size: medium; ">
<div><br>
</div>
</span><br class="Apple-interchange-newline">
</div>
<br>
</body>
</html>